ResourceLife Sciences
An Automated, High-Throughput Library Construction Protocol with Benefits for Low-Input Applications
22 Jul 2014This poster describes a versatile, automated method for high-throughput Illumina® library construction using the KAPA HTP Library Preparation Kit on the Biomek® FX Laboratory Automation Workstation (Beckman Coulter). Data presented confirms that the automated method and KAPA library construction reagents offer robust, high-throughput library construction over a range of DNA inputs and sample types, and yields sequence data comparable or better than that achieved with a highly optimized manual method, whilst employing fewer cycles of pre-capture amplification.
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Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Library PreparationLibrary preparation is a critical step in sequencing workflows, where DNA or RNA samples are converted into libraries for high-throughput, next generation sequencing. This step ensures accurate results and minimizes biases. Explore library preparation kits in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.