Airyscanning: A Novel Approach to Confocal Imaging

21 Nov 2014

Confocal laser scanning microscopy is the recognized standard for 3D fluorescence microscopy. This application note describes how Airyscanning releases the full potential of a confocal microscope, achieving resolutions comparable to an extremely small pinhole but with a much better signal-to-noise ratio. It combines excellent optical sectioning performance with flexible scanning strategies for imaging and photomanipulation, making it the method of choice for a vast range of applications.

ZEISS LSM 780 Laser Scanning Microscope

ZEISS Research Microscopy Solutions

LSM 780 - Obtain Quantitative Information About Your Cells and Individual Molecules. The sensitivity of LSM 780 is quite simply outstanding. The GaAsP detector achieves 45 percent quantum efficiency compared to 25 percent typically by conventional PMT detectors. This results in accurate details and contrast-rich images of the challenging specimens you encounter in your live cell imaging.The system’s illumination and detection design allows you to simultaneously acquire up to ten dyes. You excite any common fluorophore with up to eight different lasers, detecting the signals with the 32 channel GaAsP detector. LSM 780 is so sensitive, the system even allows photon counting.The maximum possible acquisition speed available to you is determined by the sensitivity of your detection system. When you choose LSM 780, you benefit from new GaAsP detectors that bring a significant improvement on signal-to-noise ratio, allowing you to acquire your image sequences at least twice as fast.Parallel spectral detection also reads 34 channels simultaneously in Lambda mode: you acquire and separate up to ten colors simultaneously.LSM 780 allows you to achieve images of unparalleled detail, even from the most challenging specimens. The superior quality and sensitivity has been recognized by a distinguished R&D 100 Award in 2011.LSM 780 Laser Scanning Microscope Features: 32-channel GaAsP detector with a typical quantum efficiency of 45% – a significant improvement on the 25% typically achieved by classic PMT detectors. Potential for photon counting on GaAsP and the new side PMT. With lasers from 355 nm true UV to 660 nm far red, any dye will be excited optimally. Simple adaptation to changing acquisition strategies via the ZEN software’s Smart Setup. Improved ZEN software with modules for e.g. correlative microscopy, deconvolution and time lapse. Improved dynamics for better visualization of weak signals. Active cooling together with oversampling and photon counting modes deliver improved signal-to-noise ratio.

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Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell ImagingCell imaging can be achieved using a number of techniques including confocal microscopy, transmission electron microscopy, atomic force microscopy, and light sheet microscopy.MicroscopyMicroscopy is a technique used to observe small objects in detail, from cells to materials, using light or electron microscopes. It enables researchers to examine structures with high resolution, aiding in fields such as biology, medicine, and materials science. With advanced microscopy techniques, scientists can gain insights into cellular processes, tissue structures, and material properties. Explore the best microscopy solutions in our peer-reviewed product directory, compare products, read customer reviews, and get pricing directly from manufacturers.
Airyscanning: A Novel Approach to Confocal Imaging