Advancing polyploid assembly and large-genome assembly in plants through long-read sequencing

In this application note, Novogene discusses the importance of high-quality reference genomes for scientific research, as they serve as the foundational basis for a wide range of genetic studies, including gene function, expression, evolution, genetic variation, and comparative genomics across species. The note highlights the historical challenges in constructing large and complex genomes, particularly with older sequencing methods like Sanger sequencing and next-generation sequencing (NGS) producing short reads that hindered accurate assembly. Challenges such as GC-rich regions, tandem repeats, and interspersed genes, are difficult to represent accurately with short reads. Addressing this, Novogene discusses how long-read sequencing technology can be utilized to generate extended reads that span multiple genes and chromosomes, facilitating precise gene placement.