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Advanced HPLC Size-Exclusion Chromatography for the Analysis of Macromolecular Proteins Using 3.5 μm Ethylene Bridged Hybrid (BEH) Particles

Waters
15 Dec 2014

This note will highlight the performance characteristics of both the 200Å and 450Å pore-size versions of these columns, designed for the separation of macromolecular proteins, with respect to resolution, column-to-column reproducibility, and column stability. Additionally, the distinct advantages in terms of resolution and sample-throughput of the sub-4-µm packing material over larger (5 and 8 µm) standard HPLC particle sizes for the separation of large proteins is shown.

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Waters Alliance™ HPLC System

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An integrated HPLC system that works as hard as you do, available with push-button mass detection for the chromatographer. The Alliance™ HPLC System is the flexible and reliable workhorse that meets your fundamental HPLC separation requirements. The system has been continually expanded and perfected to accommodate an enormous range of real-world analytical challenges, culminating in today’s new industrial design which signifies Waters’ commitment to HPLC and our HPLC line. Today, users can extend the Alliance HPLC System’s performance with XP 2.5 µm Columns. XP Columns are compatible with both HPLC and UPLC technology and are designed to not only improve our HPLC productivity, but also facilitate a transfer to UPLC Technology platforms as well. Directly scalable between UPLC and HPLC particle sizes, these new 2.5 um XP Columns bridge the gap between HPLC and UPLC performance. Whether your lab analyzes samples in the pharmaceutical, chemical, food safety, environmental, or quality control arenas, the Alliance HPLC system offers you a field-proven solution. Integrated solvent and sample management to ensure consistent system-to-system performance and high reproducibility. Waters Alliance™ HPLC System Features: Full integration with either Empower® or MassLynx® Software for instrument control and data processing A large, intuitive LCD-based user interface allows rapid system set-up through SystemPREP routines that streamline daily start-ups Easy, tool-free maintenance resulting in maximized uptime Waters complete line of detection technologies – from routine UV/Vis detectors, to the ACQUITY QDa Detector to single, tandem and triple quadrupole mass spectrometers - extend your application capabilities

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.

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