Advanced flow cytometry handbook

11 Sept 2024

Flow cytometry is a powerful, laser-based method for analyzing the physical and chemical characteristics of individual cells in a mixed cell population. Applications where flow cytometry analysis is used include antibody discovery, immune cell function, cytokine profiling, and cell health monitoring. Compared to alternative methods, such as microscopy-based analysis, enzyme-linked immunosorbent assay (ELISA), western blotting, and mass cytometry, flow cytometry offers several key advantages which are presented here. Explore advanced flow cytometry assays and workflows and discover why flow cytometry is an essential tool in biomedical research, vaccine and drug development, and clinical diagnostics.

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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Advanced flow cytometry handbook