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Advanced Application of CD Measurement for Pharmaceutical Research

JASCO (USA)
5 Aug 2015

Human serum albumin (HSA) is the most abundant protein in blood plasma. Chirality resulting from an achiral substance interacting with a chiral substance is called induced CD. This application note presents the measurement of CD induced by the interaction of HSA titrated with 3,5-diiodosalicylic acid and subsequent analysis of the dissociation constant, using Jasco’s J-1500 CD Spectrometer.

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J-1000 Series Circular Dichroism Spectrometers

JASCO (USA)

The JASCO J-1100 is a CD Spectrometer system designed to meet most of the demands for biomolecule analysis in a compact, lower cost package.The simple yet powerful design is perfect as a workhorse for generating consistent CD data for research as well as an effective tool for teaching CD in academic environments. The JASCO J-1500 is designed to meet the most demanding of CD applications. The performance level is unparalleled and the layout is suited for adaptation to a variety of different sampling methods. With CD, LD and Absorbance provided as standard the base unit is already very capable but when combined with optional measurement modes and sampling accessories in addition to the new Simultaneous Multi-Probe (SMP) acquisition system you can unleash the power only dreamed of by earlier systems. FEATURES Wide spectral range from vacuum UV to Near-IR Standard built-in mercury lamp and optional NIST traceable standard sample for system validation High-efficiency purge capability enabling to enhanced vacuum UV measurement Extremely low stray light and high S/N ratio providing wide dynamic range High speed scanning Simultaneous Multi-probe measurements (SMP) with acquisition of up to four data channels Flexible design allowing field upgrades for different measurement modes and accessories as applications evolve Spectra Manager II or Spectra Manager CFR (For FDA regulated labs): 64 bit innovative, cross-platform Spectroscopy Software Suite for data acquisition, analysis and presentation including several methods of secondary structure calculation

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Process ChemistryProcess chemistry is an important stage of drug development for scaling-up drug production or chemical synthesis reactions. It is useful for optimizing economical and efficient drug production. Process chemistry uses reactors and pump systems as well as reagents, standards and buffers.HematologyIn Haematology / Hematology, complete blood cell counts (or full blood counts) are obtained using automated blood count analyzers to enumerate blood cell types.  Hematology also encompasses haemostasis and coagulation, thrombophilia and hemophilia, plasma viscosity and ESR analysis, hemoglobinopathies, cell morphology and haematinic measurement.Drug DeliveryDrug Delivery refers to dosage form, route of administration, formulations, technologies, and systems for transporting a pharmaceutical compound in the body as needed to safely achieve its desired therapeutic effect. Drug delivery is often approached via the biopharmaceutical or small molecule drug's formulation, but it may also involve medical devices or drug-device combination products. Considerations include instrumentation, software and services. Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.Human Serum AlbuminSpectrometrySpectrometry is a technique used to measure the mass-to-charge ratio of ions. It provides valuable information about the composition, structure, and molecular weight of compounds. Used across a variety of fields, including chemistry, biology, and environmental science, spectrometry is essential for identifying substances, analyzing mixtures, and developing new products. Explore the best spectrometry tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.PharmaceuticalsPharmaceuticals are medicinal drugs used in healthcare to diagnose, prevent, cure and treat illnesses. Pharmaceuticals that are excreted after use appear in wastewater and can have detrimental effects on the environment.Blood AnalysisThe analysis of blood is vital for many areas of life sciences and forensic investigations. Blood samples can be tested for a number of different reasons such as diagnosis, glucose levels, cholesterol and drug testing.

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