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ADP Detection in FP, TR-FRET and FI Assay Formats: Versatile Kinase/ATPase Detection Without the Use of Coupling Enzymes

BellBrook Labs

ADP Detection in FP, TR-FRET and FI Assay Formats: Versatile Kinase/ATPase Detection Without the Use of Coupling Enzymes

3 Dec 2015

The Transcreener ADP2 Assay is a single addition, homogenous assay method that directly measures ADP formation in kinase or ATPase reactions. The first generation Transcreener ADP assay, introduced in 2006 as a far red fluorescence polarization (FP) assay, was recently improved by incorporating a new antibody (ADP2) that enables a 20-fold increase in sensitivity. Together these Transcreener ADP assays have been validated for high throughput screening with diverse targets including protein, lipid and carbohydrate kinases, carboxylases, and chaperonin ATPases. This poster compares these three assays with other nucleotide detection assay methods

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Transcreener® ADP² Kinase Assays

BellBrook Labs

The Transcreener® ADP² Kinase Assay Kit universally detects ADP produced by any kinase or ATPase using a direct mix‑and‑read format, eliminating coupling steps. Available in FP, FI, and TR‑FRET formats with far‑red tracers to minimize interference, it's HTS-ready across 96‑, 384‑, and 1536‑well plates with robust Z' ≥ 0.7 performance.

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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.EnzymesEnzymes are macromolecular biological catalysts used across life science, pharmaceutical and clinical research. Various enzymes can be purchased for research applications including ligases, high fidelity polymerases, nuclease, protease, restriction enzymes, tissue dissociation enzymes and enzyme inhibitors. Find the best enzymes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.ATP MonitoringHigh ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.LipidsLipids are a diverse group of organic compounds that are insoluble in water, including fats, oils, and hormones. They are critical for cellular structure and energy storage. Explore lipid analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.KinasesTR-FRETCell Based AssaysCell-based assays use living cells to measure biological responses to various treatments or stimuli. They are essential for drug discovery, toxicology testing, and understanding cellular mechanisms. These assays are used to assess cell viability, proliferation, gene expression, and other cellular activities. Browse our peer-reviewed product directory to find the best cell-based assay tools and reagents, compare products, read customer reviews, and get pricing directly from manufacturers.FRET

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ADP Detection in FP, TR-FRET and FI Assay Formats: Versatile Kinase/ATPase Detection Without the Use of Coupling Enzymes