ResourceLife Sciences
Adaptation of a potassium channel assay to enable easier high throughput screening
3 Mar 2025Thallium-sensitive dye-based methods have made it possible to measure monovalent ion channels such as potassium channels. However, they typically employ kinetic imaging readers that are not common laboratory equipment. Here, a modified potassium channel assay is demonstrated, that can be detected with the PHERAstar FSX with comparable results to kinetic modality readers.
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Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High Throughput ScreeningIon ChannelFluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.