Acceleration of method optimization for AAV capsid purity analysis using a multi-capillary electrophoresis platform

5 Nov 2021

This application note highlights the use of the BioPhase 8800 system for accelerating method development for adeno-associated viruses (AAV). The application note demonstrates the system’s ability to perform 8 separations in parallel allowing for faster processing of samples and overall increased throughput.

BioPhase 8800 system

SCIEX

Purpose-built to target quality and efficiency, the BioPhase 8800 system enables parallel sample throughput and analysis for capillary electrophoresis characterization. 

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.VirusesViruses are microscopic pathogens that require a host cell to replicate. Understanding their structure, replication cycle, and impact on the immune system is crucial in developing effective treatments, vaccines, and diagnostic methods. Research continues to focus on emerging viral diseases and antiviral drug discovery. Explore solutions for virus research, detection, and treatment in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.
Acceleration of method optimization for AAV capsid purity analysis using a multi-capillary electrophoresis platform