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A Universal LB-MSIA Workflow Using Freedom EVO Platform for the Pre-Clinical Analysis of Therapeutic Antibodies of Differing Allotypes in Rodent Plasma

Thermo Fisher Scientific

A Universal LB-MSIA Workflow Using Freedom EVO Platform for the Pre-Clinical Analysis of Therapeutic Antibodies of Differing Allotypes in Rodent Plasma

29 Mar 2016

This application demonstrates the effectiveness of analyzing therapeutic mAbs of different allotypes from rodent plasma using the Thermo Scientific™ LB-MSIA™ workflow on the Tecan Freedom EVO® platform utilizing enhanced capacity Thermo Scientific™ MSIA™ Streptavidin EVO microcolumns. This pre-clinical bio-analytical solution is based on mass spectrometric detection, specific for the bioanalysis of humanized, fully human and chimeric therapeutic monoclonal antibodies.

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MSIA™ Microcolumns for the Freedom EVO Platform

Thermo Fisher Scientific

Next generation large molecule bioanalysis made easier by combining the Thermo Scientific™ innovative MSIA™ technology with Tecan advanced, robust and easy-to-use liquid handling. MSIA affinity microcolumns, designed for Freedom EVO platform (MCA 96 head), provide innovative enablement in the areas ranging from bioanalysis of therapeutic mAbs, antibody-drug conjugates (ADC), biotransformation [in-vivo modifications, drug-antibody ratio (DAR)], preclinical and clinical drug trials (PKPD, DMPK, and ADME) to biomarker and proteins analysis. This unique solution is designed to deliver the accuracy, reliability and automation you need to be successful.   Features: Robust Automation:  Simply program the Freedom EVO to elute the analyte from complex biological samples and let the automated platform carry the load. Highly sensitive: Analyze proteins of interest down to femtomole level. Enhanced characteristics:  Detects multiple protein variants for qualitative and quantitative bioanalysis in a single assay. Contaminant-free: proprietary molecular trapping microcolumn technology prevents matrix injection that would impact LC-MS function. High signal-to-noise ratio: Innovative sample interface technology reduces matrix loss, reagent carryover and non-specific binding. Time Savings: Microfluidic channels drive kinetic interactions significantly decreasing incubation time required to process the samples, while the MCA 96 head enables parallel processing of 96 samples. Reproducible: Consistent performance for assay reproducibility. Preserving samples: Small sample volumes (as low as 10μL) and a reduced requirement for repeating experiments. MSIA Streptavidin EVO microcolumns provide end user flexibility to analyze their specific targets of interest.

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Animal ModelsThe use of non-human animals in experiments or behavorial observations. The research is conducted inside universities, medical schools, pharmaceutical companies, farms, defence establishments, and commercial facilities that provide animal-testing services to industry. It includes pure research such as genetics, developmental biology, behavioral studies, as well as applied research such as pharmaceutical testing in pre-clinical, before human, studies. Clinical TrialsClinical Trials, an essential part of drug discovery process, assess the safety and effectiveness of a new medication or device in the pharmaceutical industry. Clinical Trials are a phased process (Phase 0, Phase I, Phase II, Phase III and Phase IV) which begins after initial preclinical testing.ImmunoassayImmunoassays are laboratory tests that utilize antibody-antigen interactions to detect specific substances. They are widely used in diagnostics for detecting diseases, drug testing, and environmental monitoring. These assays offer rapid, sensitive, and precise detection of biomarkers, making them invaluable in clinical and research labs. Browse our peer-reviewed product directory to compare immunoassay systems, read user reviews, and get pricing directly from manufacturers.Monoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.BiopharmaceuticalsBiopharmaceuticals are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or for in vivo diagnostics. The most well known example of a biopharmaceutical product, and the first to be approved for therapeutic use, was recombinant human insulin.Therapeutic AntibodiesTherapeutic antibodies are antibodies engineered for use in treating diseases. They can be designed to target specific antigens, blocking harmful interactions or modulating immune responses. Therapeutic antibodies are critical in treating conditions like cancer, autoimmune disorders, and infectious diseases. Explore the best therapeutic antibody products in our peer-reviewed product directory; compare options, check reviews, and get pricing directly from manufacturers.PlasmaBiomarker DiscoveryClinical biomarkers refer to substances related to known medical conditions that can be accurately measured <i>in vitro</i>. Biomarkers can be used to diagnose presence of a disease and indicate disease severity. The discovery of new biomarkers is incredibly valuable in the field of diagnostics.

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A Universal LB-MSIA Workflow Using Freedom EVO Platform for the Pre-Clinical Analysis of Therapeutic Antibodies of Differing Allotypes in Rodent Plasma