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A Simplified Protocol to Streamline Inhibitor Profiling with the Transcreener® ADP2 FP Assay

BellBrook Labs
13 Sept 2015

Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes - many of which catalyze the covalent regulatory reactions that are central to cell signaling and are of great value as targets in drug discovery. The Transcreener® ADP2 FP Assay is a far-red, competitive fluorescence polarization (FP) assay based on the detection of ADP and is a simple, one-step homogenous detection assay. This Application Note describes how to streamline the process of inhibitor profiling with the Transcreener® ADP2 FP Assay eliminating the need to experimentally optimize the ADP2 antibody concentration and the need for a standard curve.

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Transcreener® ADP² Kinase Assays

BellBrook Labs

The Transcreener® ADP² Kinase Assay Kit universally detects ADP produced by any kinase or ATPase using a direct mix‑and‑read format, eliminating coupling steps. Available in FP, FI, and TR‑FRET formats with far‑red tracers to minimize interference, it's HTS-ready across 96‑, 384‑, and 1536‑well plates with robust Z' ≥ 0.7 performance.

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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.High-Content ScreeningHigh-content screening (HCS), also known as high-content analysis (HCA), is a high-throughput technique used in drug discovery to identify substances that alter the phenotype of cells. HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.EnzymesEnzymes are macromolecular biological catalysts used across life science, pharmaceutical and clinical research. Various enzymes can be purchased for research applications including ligases, high fidelity polymerases, nuclease, protease, restriction enzymes, tissue dissociation enzymes and enzyme inhibitors. Find the best enzymes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.Targeted ScreeningCell Based AssaysCell-based assays use living cells to measure biological responses to various treatments or stimuli. They are essential for drug discovery, toxicology testing, and understanding cellular mechanisms. These assays are used to assess cell viability, proliferation, gene expression, and other cellular activities. Browse our peer-reviewed product directory to find the best cell-based assay tools and reagents, compare products, read customer reviews, and get pricing directly from manufacturers.ScreeningUsing robotics, data processing and control software, liquid handling devices and sensitive detectors, screening allows a researcher to quickly conduct millions of chemical, genetic or pharmacological tests.Cell Signaling

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A Simplified Protocol to Streamline Inhibitor Profiling with the Transcreener® ADP2 FP Assay