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A Simplified Protocol to Streamline Inhibitor Profiling with the Transcreener® ADP2 FP Assay

BellBrook Labs
27 Jan 2015

Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes - many of which catalyze the covalent regulatory reactions that are central to cell signaling and are of great value as targets in drug discovery. This Application Note describes how to streamline the process of inhibitor profiling with the Transcreener® ADP2 FP Assay by eliminating the need to experimentally optimize the ADP2 Antibody concentration and the need for a standard curve.

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Transcreener® ADP² Kinase Assays

BellBrook Labs

The Transcreener® ADP² Kinase Assay Kit universally detects ADP produced by any kinase or ATPase using a direct mix‑and‑read format, eliminating coupling steps. Available in FP, FI, and TR‑FRET formats with far‑red tracers to minimize interference, it's HTS-ready across 96‑, 384‑, and 1536‑well plates with robust Z' ≥ 0.7 performance.

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