ResourceSeparations
A novel screening approach for comparing LC-MS reversed-phase and HILIC methods for separations in biological matrices using amino acid examples
17 May 2023In this application note, Advanced Materials Technology presents experimental data on a novel screening approach for comparing LC-MS reversed-phase and HILIC methods for separations in biological matrices using amino acid examples. RPLC separations were performed on a HALO® 90 Å AQ-C18, 2.7 µm, 2.1 x 150 mm column. Download this free application note to find out the results and conclusions of these analyses.
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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein SeparationHILIC