ResourceSeparations
A guide to two-dimensional gas chromatography
26 Mar 2020Comprehensive two-dimensional gas chromatography (GC×GC) is an advanced analytical technique with an increased separation capacity suitable for the analysis of complex samples. This application note demonstrates some of the most important applications of two-dimensional gas chromatography.
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Flash ChromatographyFlash chromatography is a fast-preparative column chromatography technique used for separation and purification of liquid analytes. Flash chromatography systems use small silica gel particles for separation and a pressurized gas for driving the solvent through the column or pre-packed cartridge. Find the best flash chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gas ChromatographyGas chromatography (GC) is an analytical technique used to separate and quantitate mixtures of small and volatile compounds. Gas chromatographs or GC systems include components such as GC columns, detectors, pumps and autosamplers. Choose from packed or capillary GC columns, flame ionization (FID), photoionization (PID) electron capture detectors and selective or non-selective detectors. Find the best gas chromatographs in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Preparative ChromatographyPreparative chromatography equipment is used for purification purposes, separating and collecting compounds eluted from a mixture which can then be subsequently used for further purposes. This can be done in large quantities or small. Preparative chromatography systems are usually low to medium pressure liquid chromatographs that are semi-automated. Explore a range of preparative columns and fraction collectors. Find the best preparative chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Supercritical Fluid ChromatographySupercritical Fluid Chromatography (SFC) is used for the rapid separation and analysis of complex mixtures. SFC systems require injectors, pumps, columns, ovens and detectors. The SFC retention method uses a supercritical fluid, usually CO2. There are a number of different SFC detector types including UV / VIs, ECD, mass spectrometry and fluorescence.Thin Layer ChromatographyThin-layer chromatography (TLC), also called planar chromatography, is an inexpensive and simple liquid chromatographic technique used to separate and identify small amounts of compound in a mixture, monitor progress of a reaction or determine purity of substance. TLC plates come in multiple sizes with common adsorbent layers being silica, aluminum oxide or cellulose and high-performance (HP-TLC) plates are also available for increased sensitivity. Other equipment needed includes TLC developing chambers, applicators, TLC sprayers and visualizing devices, such as a viewing cabinet and UV lamp. Find the best TLC products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.GC-MS GC-MS (gas chromatography-mass spectrometry) instruments and equipment are used to separate, quantify and identify mixtures of small and volatile compounds, such as polycyclic aromatics, fatty acids and alcohols. Often used in drug detection, forensic investigation and environmental analysis for pesticides and contaminants, GC-MS is a powerful addition to your lab’s analytical capabilities. GC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzers can give greater sensitivity and resolution to your analysis. Find the best GC-MS instruments and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.UltraPerformance Convergence ChromatographyConvergence Chromatography expands the capabilities of reversed phase LC and conventional GC techniques. By varying mobile phase strength, pressure, temperature and stationary phase Convergence Chromatography facilitates the separation, detection and quantification of many compounds and samples. The CO<sub>2< / sub> mobile phase used reduces the need for toxic solvents.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.2D ChromatographyGas ChromatographyGas chromatography (GC) is an analytical technique used to separate and analyze gases or volatile liquids in a sample. It is widely used in environmental testing, food safety, pharmaceuticals, and forensic science. GC is ideal for identifying and quantifying compounds that are gaseous or can be vaporized without decomposition. Browse our peer-reviewed product directory to find the best gas chromatography systems, compare products, check reviews, and get pricing directly from manufacturers.