A Guide to Biological Buffer Preparation for pH Measurement and Pipetting

Researchers in Life Sciences carry out work on biological molecules, such as antibodies or proteins, in order to modify them for their purposes, often in the search for disease cures and prevention. Almost all biological processes are pH dependent. The purpose of buffer in a biological system is to maintain intracellular and extracellular pH within a very narrow range and resist changes in pH in the presence of internal and external influences. The rather complex laboratory buffer preparation process involves a number of different steps, including amount calculation, substance weighing, pH solution value control and liquid pipetting. This application note explains some useful tips and hints for the use of pipettes and pH Meters in the preparation of buffers.