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A Fully Automated, Bottom-up Approach for MALDI-TOF MS Based Discovery Workflows

Shimadzu Corporation
31 Aug 2015

This application note demonstrates a fully automated solution for MALDI-TOF MS based peptide mapping experiments. Using an online digestion, desalt, reversed phase separation and fraction collection platform, the average experimental time was decreased from over 18 hours to less than 30 min (reversed phase separation included – acquisition time not included) while substantially improving sequence coverage.

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AccuSpot

The AccuSpot automates LC microfractionaction, spotting and preparation for MS analysis. With the AccuSpot system, LC eluent can be accurately and continuously spotted onto target plates, in trace amounts of 50nL or more. This allows for automatic preparation of target plated for MALDI-TOF-MS measurements. By using it in conjunction with Shimadzu's 1D or 2D Micro LC System and Shimadzu Biotech's AXIMA MALDI-TOF instruments, proteome analysis can be performed with a higher degree of precision and with greater sequence coverage.

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. MALDI-TOF MSMALDI-TOF mass spectrometers (matrix-assisted laser desorption/ionization time-of-flight) are used for the mass analysis of large molecules, including proteins, DNA, polymers and other macromolecules due to its relatively soft ionization, reducing fragmentation whilst allowing fast data acquisition. Tandem mass analyzers such as TOF/TOF systems can be used to increase resolution and sensitivity of your analysis. Find the best MALDI-TOF products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Discovery SoftwareComputational techniques used in both the chemistry and biology aspects of drug discovery, for data acquisition, processing and storage. Software is used for analysis of pathways, target identification, genomics, proteomics, modelling, lead identification, compound analysis and drug modelling among many others. Digestion and ExtractionDigestion and extraction apparatus are used in sample preparation to break down samples or extract organic or aqueous compounds from a solid. Digestion may be conducted via hot block, microwave or Kjeldahl digestion apparatus. Extraction systems typically involve solvent extraction and include Soxhlet, Randall or Twisselmann extraction methods. Find the best digestion and extraction apparatus in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Peptide MappingSequencingSequencing refers to determining the order of nucleotides in a DNA or RNA molecule. This process is essential in genomics, diagnostics, and evolutionary biology. Techniques like next-generation sequencing (NGS) and Sanger sequencing provide high-throughput and precise data for analyzing genomes and transcriptomes. Browse our peer-reviewed product directory to find the best sequencing tools and reagents, compare products, check reviews, and get pricing directly from manufacturers.Reversed-Phase LC

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