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A Fast and Robust HPLC Separation of Bile Acids, and their Conjugates, with Ascentis® Express C18
Helping to Elucidate the Role of Plasma Bile Acid Signalling in Humans
18 Jan 2016This application note from Sigma Aldrich describes how HPLC can be used to separate Bile acids and their conjugates. Bile acid has an important role in control of the immune system which has led to research interest in their levels in the blood plasma. This application note features the Ascentis® Express C18 from Sigma Aldrich which can be seen here.
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Ascentis® Express C18
Sigma-Aldrich SupelcoC18 HPLC column for High Speed and High Resolution Applications. Ascentis® Express C18 is the first choice for starting a new method. Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles at lower system pressure. With identical conditions, the Ascentis Express C18 column performs more than 4X faster than a standard C18 column, for most applications. The combination of high efficiency and lower operating pressure benefits UHPLC (or other ultra high pressure system) users, as well as conventional HPLC users. The increased efficiency of Ascentis Express columns enables you to use shorter columns and reduce solvent usage/cost. The Ascentis® Express C18 is also featured in an application note about liquid GC columns which can be found on the SelectScience website here, and a study on HPLC separation of bile acid here. Column details: Mode: Reversed Phase USP Code: L1 Particle Size: 2.7, <2.0 μm Pore Size: 90 Å Length: 20, 30, 50, 75, 100, 150 mm ID: 1.0, 2.1, 3.0, 4.6, 10 mm
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