SelectScience
Join Free
Accelerating Science
ResourceSeparations

A direct IEX-MS method for the detection of mAb charge variants

Waters
25 Mar 2020

This application note demonstrates a direct IEX-MS method with broad utility for the successful detection of mAb charge variants. Platform methods can be easily developed using the BioResolve SCX mAb Column, IonHance CX-MS pH Concentrates, and BioAccord LC-MS System which provide robust and reproducible separations along with high quality mass spectra in the elucidation of various mAb-charge variant species.

Related products

Request Quote for All Products

BioAccord LC-MS System

Waters

The First SmartMS-Enabled Biopharma Solution  The Waters BioAccord System is an integrated system that simplifies high performance LC-MS biopharmaceutical analysis for every user. An easy-to-use system solution that puts the power to make decisions directly in your hands; a self-calibrating, self-optimizing, self-sufficient tool that equips you with high quality data you can use to tackle the challenges you face every day during biopharmaceutical development.

(15)

UNIFI Scientific Information System

Waters

Waters UNIFI Scientific Information System is the first software platform to merge LC and high performance MS data (both quadrupole and time-of-flight) into a single solution that encompasses data acquisition, processing, visualization, reporting, and configurable compliance tools within a networked laboratory environment.

(4)

BioResolve Columns

Waters

More than just a column -  A solution providing application-focused standards, methods, and exclusive support so you can easily and reliably achieve state-of-the-art separations.

(15)

Links

WatersCompany website

Tags

Capillary ElectrophoresisCapillary electrophoresis (CE) is used to separate molecules based on charge, size and hydrophobicity. Pressure, voltage or a vacuum is used to introduce the sample to fused silica capillaries. Fluorescence, UV/Vis, UV or diode array detectors are used to visualize components. Types of CE systems include capillary electrochromatography (CEC), capillary zone electrophoresis and capillary gel electrophoresis. Find the best capillary electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.Capillary Ion ChromatographyElectrophoresisBiopharmaceuticalsBiopharmaceuticals are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or for in vivo diagnostics. The most well known example of a biopharmaceutical product, and the first to be approved for therapeutic use, was recombinant human insulin.

Related resources