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A customized XF workflow for detection and characterization of mitochondrial toxicity

Agilent Technologies
22 Feb 2023

Learn more about the Agilent Seahorse XF Mito Tox Assay, a customized XF solution that allows the detection and quantitative assessment of compounds eliciting mitochondrial toxicity. This application note provides proof of concept and examples for compound screening, dose-response experiments using the XF Mito Tox assay, and interpretation of resulting data using the Mito Tox Index (MTI). It also presents a discussion of strategic assay design, performance metrics, and specific cases when using the XF Mito Tox Assay.

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Seahorse XF Pro Analyzer

Agilent Technologies

Measure mitochondrial respiration, glycolysis, and ATP production rates in live cells in a 96-well forma t, providing a system-level view of cellular energy metabolism and mitochondrial health. It combines enhanced sensitivity and data consistency with intuitive software and advanced data analytics, enabling users to streamline assay workflow from start to finish and generate and interpret metabolic data easily. 

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Data AnalysisData analysis hardware and software is available to make data processing straight-forward yet powerful. Data software can be used for math and stats, technical graphing and image analysis. In addition, software is available for specific data analysis of electrophoresis, densitometry, ELISA and DNA sequencing.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.EnzymesEnzymes are macromolecular biological catalysts used across life science, pharmaceutical and clinical research. Various enzymes can be purchased for research applications including ligases, high fidelity polymerases, nuclease, protease, restriction enzymes, tissue dissociation enzymes and enzyme inhibitors. Find the best enzymes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MitochondriaReporter AssayCompound ScreeningCompound screening is a method used to discover specific compounds that could be promising candidates for pharmaceutical use. This potential is identified when compounds interact with the target protein during screening and could therefore be carried forward in the drug development process.

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A customized XF workflow for detection and characterization of mitochondrial toxicity