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6 Color Panel for Staining Human Peripheral Blood Lymphocytes
6 Color Panel for Staining Human Peripheral Blood Lymphocytes
20 Nov 2015This application note describes immunophenotypic analysis of human peripheral blood lymphocytes utilizing a 6-color antibody panel in a stain/lyse protocol run on the CytoFLEX Research Flow Cytometer. The markers chosen are commonly used surface CD antigens which can be used to quantify and characterize normal and abnormal lymphocyte populations in peripheral blood.
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CytoFLEX Flow Cytometer
Beckman Coulter Life SciencesProviding quality and performance at any configuration, the CytoFLEX system provides powerful sensitivity and resolution for the simple to the most challenging applications. CytoFLEX delivers and surpasses capabilities expected in top tier analyzers, with excellent performance and nanoparticle resolution.
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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.ImmunoassayImmunoassays are laboratory tests that utilize antibody-antigen interactions to detect specific substances. They are widely used in diagnostics for detecting diseases, drug testing, and environmental monitoring. These assays offer rapid, sensitive, and precise detection of biomarkers, making them invaluable in clinical and research labs. Browse our peer-reviewed product directory to compare immunoassay systems, read user reviews, and get pricing directly from manufacturers.LymphocytesImmunophenotypingBlood AnalysisThe analysis of blood is vital for many areas of life sciences and forensic investigations. Blood samples can be tested for a number of different reasons such as diagnosis, glucose levels, cholesterol and drug testing.