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3D Spheroid Culture and Drug Testing Using a Novel Hanging Drop Culture Plate

3D Biomatrix
26 Aug 2013

3D cell culture systems are essential to acquiring physiologically‐relevant results. However, scaling up and maintaining reproducibility of 3D cell cultures have been difficult due to lack of standardized tools and methods that are simple to use and compatible with existing equipment. This scientific poster present a novel 3D cell culture platform that enables uniformly-sized spheroids to be generated and tested in a 384-microplate format and using high throughput screening instruments, such as plate readers, liquid handling robots, and automated imaging and analysis systems.

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Perfecta3D Hanging Drop Plates - 384-Well

3D Biomatrix

The patent-pending Perfecta3D® Hanging Drop Plates are 96- and 384-well plates that facilitate the formation, culture, and testing of three-dimensional (3D) spheroids and stem cell aggregates without the aid of coatings or matrices. With only one spheroid forming in each well, 3D spheroids formed in Perfecta3D Hanging Drop Plates are consistent and diameter can be controlled by the number of cells seeded in each well. Media and compounds can be added or removed from the top of the plate, and cells can be added for patterned co-cultures. The well plate format means that the plates are compatible with manual or automated liquid handling equipment. Product and ordering information can be found at www.3DBiomatrix.com/products.

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Perfecta3D Hanging Drop Plates - 96-Well

3D Biomatrix

The patent-pending Perfecta3D® Hanging Drop Plates are 96- and 384-well plates that facilitate the formation, culture, and testing of three-dimensional (3D) spheroids and stem cell aggregates without the aid of coatings or matrices. With only one spheroid forming in each well, 3D spheroids formed in Perfecta3D Hanging Drop Plates are consistent and diameter can be controlled by the number of cells seeded in each well. Media and compounds can be added or removed from the top of the plate, and cells can be added for patterned co-cultures. The well plate format means that the plates are compatible with manual or automated liquid handling equipment. Product and ordering information can be found at www.3DBiomatrix.com/products.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample ManagementSample management systems include sample storage devices such as freezers and plate storers, sample environment enclosures and sample organization, retrieval and sorter systems. Useful system features include high-throughput, automation, robotic arms, automated liquid handling and associated database systems. Accessories in sample management include barcode scanners, heat sealers and tubes.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.High-Content ScreeningHigh-content screening (HCS), also known as high-content analysis (HCA), is a high-throughput technique used in drug discovery to identify substances that alter the phenotype of cells. HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Robotic WorkstationsRobotic workstations are automated platforms enabling the user to perform high-throughput, multi-application processes, from sample preparation tasks such as PCR set-up, DNA purification and NGS library preparation, to integrated sample prep and analysis workstations. Automation makes processes consistent, fast, precise and fully walk-away. Find the best robotic workstations in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Drug TestingIt is essential to test the efficacy and toxicity of drugs at every stage of their development to ensure that a pharmaceutical product is fit for purpose and safe for use before reaching the stage of mass production and distribution.ScreeningUsing robotics, data processing and control software, liquid handling devices and sensitive detectors, screening allows a researcher to quickly conduct millions of chemical, genetic or pharmacological tests.Compound ScreeningCompound screening is a method used to discover specific compounds that could be promising candidates for pharmaceutical use. This potential is identified when compounds interact with the target protein during screening and could therefore be carried forward in the drug development process.Screening System

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3D Spheroid Culture and Drug Testing Using a Novel Hanging Drop Culture Plate