3D spheroid-based tumor invasion assay

The establishment of in vitro models that mimic tumor invasion as part of the metastatic process is a critical part of oncology research. The need to incorporate multiple cell types, long-term kinetic analysis, methods to allow 3D invasion into the surrounding matrix, and appropriate detection and analysis, has made it difficult to create new models. In this application note, learn more about the procedure that meets these needs through the inclusion of advanced imaging capabilities allowing for the capture of multiple images throughout a range of z-heights, using brightfield and fluorescence channels in a kinetic fashion.