3D High-Content Screening of Liver Spheroids
9 Apr 2018More accurate in vitro toxicity assays are required to detect adverse drug effects, such as drug induced liver injury (DILI), in earlier phases of drug development. 3D cell models demonstrate the formation of functional bile canaliculi and have increased albumin secretion and CYP expression, as well as longer stability in culture compared with two dimensional (2D) cultures, making them ideal for DILI studies. Here, efficacious drug screening is demonstrated by combining Corning® Hepatocells with Corning spheroid microplates to create the 3D cell cultures necessary for testing.
Related products
Request Quote for All Products
Corning® 384-well Black/Clear Round Bottom Ultra-Low Attachment Spheroid Microplate, Bulk Packed 10 per Bag, with Lid, Sterile
Corning Life SciencesUltra-Low Attachment microplates feature a covalently bound hydrogel layer that effectively inhibits cellular attachment Surface minimizes protein absorption, enzyme activation and cellular activation Surface is noncytotoxic, biologically inert and nondegradable Sterilized by gamma irradiation Black wall microplates have low background fluorescence, minimal light scatter and reduced crosstalk Opaque walls to prevent well-to-well crosstalk Can be used for both top and bottom reading instruments Bulk packed 10 per bag Novel well geometry aids formation of spheroids in center of well Optically clear round bottom with black opaque microplate body
Corning® 96-well Black/Clear Round Bottom Ultra-Low Attachment Surface Spheroid Microplate, Bulk Packed, with Lid, Sterile, 10/Bag
Corning Life SciencesUltra-low attachment surface with covalently bonded hydrogel that minimizes cell attachment, protein absorption, enzyme activation and cellular activation The surface is noncytotoxic, biologically inert and nondegradable Round bottoms with 300 µL total volume Recommended working volumes of 0.075 to 0.30 mL Sterilized by gamma radiation Novel well geometry aids formation of spheroids in center of well Optically clear round bottom with black opaque microplate body