10x Genomics: Demystifying single-cell sequencing

18 Oct 2021

In order to advance discovery, scientists need tools that can provide a comprehensive view of the underlying biological complexity of their samples. For a growing number of researchers, single-cell RNA-sequencing and single-cell multi-omics—genomic approaches for quantitative analysis of gene expression and other DNA, RNA, or protein-based analytes at single-cell resolution—are proving effective to address their biological questions. Download this application book to find out how 10X Genomics is tackling the issues of the future of single-cell sequencing.

Chromium Controller

10x Genomics

Chromium Controller uses advanced microfluidics to perform single cell partitioning and barcoding in a matter of minutes. Powered by Next GEM technology, Chromium Controller enables integrated analysis of single cells at massive scale. Our suite of Chromium Single Cell products can capture molecular readouts of cell activity in multiple dimensions, including gene expression, cell surface proteins, immune clonotype, antigen specificity, and chromatin accessibility.  PRODUCT FEATURES Cell by cell characterization: Go beyond population averages to capture and resolve cellular heterogeneity. Rapid single cell profiling: Partition hundreds to tens of thousands of nuclei or cells per chip in minutes. Compact and flexible: Small instrument footprint easily integrates into existing laboratory infrastructure.

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Chromium Single Cell Gene Expression

10x Genomics

Chromium Single Cell Gene Expression provides single cell transcriptome 3' gene expression and multiomic capabilities to profile tens of thousands of cells. Explore cellular heterogeneity, novel targets, and biomarkers with combined gene expression, surface protein expression, or CRISPR edits in each cell.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.SequencingSequencing refers to determining the order of nucleotides in a DNA or RNA molecule. This process is essential in genomics, diagnostics, and evolutionary biology. Techniques like next-generation sequencing (NGS) and Sanger sequencing provide high-throughput and precise data for analyzing genomes and transcriptomes. Browse our peer-reviewed product directory to find the best sequencing tools and reagents, compare products, check reviews, and get pricing directly from manufacturers.Single Cell Genomics
10x Genomics: Demystifying single-cell sequencing