Gene Expression and Molecular Cloning > mRNA Transcription Products & Reviews

Specially formulated for high yields of canonical (GAUC) in vitro transcribed RNA. Produce up to 90 µg of RNA from 1 µg of template in 2 hours. Easily scale up reactions to produce milligram amounts of RNA.

Amplify micrograms of circular DNA from as little as 5 pg of input. Directly amplify from purified plasmid, glycerol stocks, liquid media cultures, or colonies on agar plates. Accurate amplification, with an error rate of ~1 in 106-107 bases.

Build Cap 0 caps with nearly 100% efficiency. Use simultaneously with ScriptCap™ 2'-O-Methyltransferase to produce Cap 1 caps.

Co-transcriptionally cap in vitro transcribed RNA with Anti-Reverse cap analog (ARCA). Produce up to 60 µg of capped RNA from 1 µg of template in just 30 minutes. Optimized for maximum transcript capping and yield.

Generate N1-methyl-pseudouridine-containing mRNA (N1meΨ-mRNA) with greatly reduced double-stranded RNA (dsRNA) content. Easily remove dsRNA without the need for chromatographic methods like HPLC. Produce large amounts of N1meΨ-mRNA with virtually 100% transcript capping. Induce lower innate immune response in mammalian cells.

Remove cap structures (decapping) from various RNAs. Substitute unit for unit into any prexisting protocol for Tobacco Acid Pyrophosphatase (TAP).

Build Cap 0 or Cap 1 caps with nearly 100% efficiency. Provides all reagents for capping in vitro transcribed RNA.

Prepare Cap 1-RNA from any source of Cap 0-RNA. Use simultaneously with ScriptCap™ m7G Capping System to produce virtually 100% Cap 1-capped RNA in a single reaction.

Generate pseudouridine-containing mRNA (Ψ-mRNA) with greatly reduced double-stranded RNA (dsRNA) content. Easily remove dsRNA without the need for chromatographic methods like HPLC. Produce large amounts of Ψ-mRNA with virtually 100% transcript capping. Induce lower innate immune response in mammalian cells.

Optimized for high-yield synthesis of 5-methyl-cytidine- pseudouridine-containing, in vitro transcribed RNA (5mCΨ-RNA). Produce up to 100 µg of 5mCΨ-RNA from 1 µg of template in 2 hours. Easily scale up to produce milligram amounts of 5mCΨ-RNA. Induce lower innate immune responses in mammalian cells by using 5mCΨ-mRNAs.

Quantitative mRNA poly(A) tail length using standard polyacrylamide gel electrophoresis (PAGE). Easy, cost-effective benchtop method for QC as an alternative to mass spec analysis. Assess tail lengths above the limits of HPLC and LC-MS based methods.

Quantitate mRNA capping efficiency (percent capped RNA content) of XBG 5’ UTR-containing mRNA using standard polyacrylamide gel electrophoresis (PAGE). XBG 5’ UTR Targeting Oligonucleotide and XBG 5’ UTR Control Mix included in kit. Easy, cost-effective, benchtop method useful for QC as an alternative to HPLC and LC-MS analysis.

Easy to use, scalable enzymatic method for removing double-stranded RNA (dsRNA) from RNA samples. Remove dsRNA without the need for chromatographic methods like HPLC. No reduction in single-stranded RNA (ssRNA) yields. Reduces the innate immunogenic response to mRNA in cells.

Inhibits eukaryotic RNases A, B and C. Use as a safeguard against RNA degradation in any RNA-containing reaction.

Optimized for high-yield synthesis of pseudouridine-containing, in vitro transcribed RNA (Ψ-RNA). Produce up to 100 µg of Ψ-RNA from 1 µg of template in 2 hours. Induce lower innate immune responses in mammalian cells by using Ψ-mRNA.

All-in-one kit to produce N1-methyl-pseudouridine-containing-mRNA (N1meΨ-mRNA). Combine the benefits of high-yield transcription with post-transcriptional capping and tailing. Produce large amounts of N1meΨ-mRNA with virtually 100% transcript capping. Induce lower innate immune response in mammalian cells by using N1meΨ-mRNAs.

Digests linear RNA and Y-structure RNA, leaving circRNA, lariat intron RNA, and dsRNA intact. Ideal for isolating circRNA from total RNA preparations. 1 µg of RNase R digests 5 µg of linear RNA in 30 minutes.

Specially formulated for maximum yields of pseudouridine-containing, in vitro transcribed RNA (Ψ-RNA). Produce up to 160 µg of Ψ-RNA from 1 µg of template in 1 hour. Easily scale up to produce milligram amounts of Ψ-RNA. Induce lower innate immune responses in mammalian cells by using Ψ-mRNAs.

Optimized for high-yield synthesis of pseudouridine-containing, in vitro transcribed RNA (Ψ-RNA). Produce up to 60 µg of Ψ-RNA from 1 µg of template in 2 hours. Easily scale up to produce milligram amounts of Ψ-RNA. Induce lower innate immune responses in mammalian cells by using Ψ-mRNA.

For post-transcriptional poly(A) tail addition to RNAs. Tail mRNAs in vitro to lengths equivalent to or greater than those found in nature.

Co-transcriptionally cap 5-methyl-cytidine- pseudouridine-containing, in vitro transcribed RNA with Anti-Reverse cap analog (ARCA). Produce up to 40 µg of capped 5mCΨ-RNA from 1 µg of template in 3 hours. Optimized for maximum transcript capping and yield. Induce lower innate immune responses in mammalian cells by using 5mCΨ-mRNAs.

Quantitate mRNA capping efficiency (percent capped RNA content) using standard polyacrylamide gel electrophoresis (PAGE). Easy, cost-effective, benchtop method useful for QC as an alternative to HPLC and LC-MS analysis.

Quantitate the percentage of mRNA with Cap 1 vs Cap 0 5’-cap structures using standard polyacrylamide gel electrophoresis (PAGE). Easy, cost-effective benchtop method for QC as an alternative to mass spec analysis.

All-in-one kit to produce pseudouridine-containing-mRNA (Ψ-mRNA). Combine the benefits of high-yield transcription with post-transcriptional capping and tailing. Produce large amounts of Ψ-mRNA with virtually 100% transcript capping. Induce lower innate immune response in mammalian cells by using Ψ-mRNAs.

Specially formulated for maximum yields of N1-methyl-pseudouridine-containing, in vitro transcribed RNA (N1meΨ-RNA). Produce up to 160 µg of N1meΨ-RNA from 1 µg of template in 1 hour. Easily scale up reactions to produce milligram amounts of N1meΨ-RNA. Induce lower innate immune responses in mammalian cells by using N1meΨ-mRNAs.