Western Blotting Products & Reviews

GenScript has developed a series of rabbit monoclonal (MonoRab™) anti-VHH antibodies to facilitate your research of VHHs.

Biotin labelling of protein, amine reactive.  Only 1 hour to get conjugates, all processes in a single filtration tube, high recovery of conjugates, applicable for 50-200 ug IgG

Our Chemifluorescence Western Blotting kit allows sensitive, Chemifluorescent detection of specific Protein targets. Attomolar detection sensitivity Non-isotopic detection with low background Quantitation over four orders of magnitude Optimized for use with the FMBIO® Fluorescence Imaging System During a chemifluorescent assay, a nonfluorogenic substrate is coverted to a fluorescent product by an enzyme such as alkaline phos…

These Semi Dry Blotters offer rapid transfer times for DNA, RNA and protein blotting, typically 15 to 30 minutes. All units can be used for all types of blotting: western, southern and northern via uncomplicated buffer and set up procedures and are compatible with gel thicknesses from 0.25 up to 10mm without the need for additional equipment. Each unit is compatible with their respective omniPAGE vertical gel system. Semi Dry…

These Capillary Blotting systems offer a contained unit for southern and northern blotting. These units incorporate optimized permanent wicks which eliminate the requirement for overlapping wicks, offering greater consistency. The porous polyethylene platform draws up transfer buffer faster than traditional systems allowing for faster transfer times. Side by side transfer of multiple gels can be performed in a single set up. A…

Four different sample number size and style of Hybridisation Manifold are offered: two types of dot blotter and two types of slot blotter.Typical applications include clone screening with DNA / RNA probes in Southern / Northern blots and immunological screening with antibodies in western blots. The units incorporate precision lapped surfaces to ensure uniform blotting membrane contact and a leak proof gasket. These prevent lat…

ApplicationThe kit is used for the quantitative determination of BrdU incorporated into cellular DNA using a 96-well microplate cell ELISA format. Benefits Safer: No radioisotopes are used. Accurate: The results strongly correlate to those obtained with the [3H]-thymidine method. Sensitive: As sensitive as [3H]-thymidine. Fast: The use of multiwell ELISA readers allows for processing of a large number of samples. Ea…

I-Block™ reagent is a highly purified casein-based blocking reagent.

The protein-free Blocking Buffer is a ready-to-use solution intended for blocking of the remaining free binding sites after membrane coating. The blocking is highly effective and stable.Our Synthetic Blocking Buffer™ eliminates the risk of false positive cross reactions in the assay. Current safety hazards related to the use of bovine material, for instance BSA are avoided. The buffer is ready-to-use and offers a high level of…

OPD, Ortho-Phenylenediamine, is used as a chromogen substrate in ELISA procedures with an absorption maximum at 492 nm and produces a yellow colour during the enzymatic degradation of hydrogen peroxide by horseradish peroxidase. After addition of sulfuric acid you will obtain a very stable end solution. The fizzing, buffer containing tablets are dissolved in distilled water within few minutes. This will give solution of pH 5.2…

OPD, Ortho-Phenylenediamine, is used as a chromogen substrate in ELISA procedures with an absorption maximum at 492 nm and produces a yellow colour during the enzymatic degradation of hydrogen peroxide by horseradish peroxidase. After addition of sulfuric acid you will obtain a very stable end solution. The fizzing, buffer containing tablets are dissolved in distilled water within few minutes. This will give solution of pH 5.2…

OPD is used as a chromogen substrate in ELISA procedures with an absorption maximum at 492 nm and produces a yellow colour during the enzymatic degradation of hydrogen peroxide by horseradish peroxidase. After addition of sulfuric acid you will obtain a very stable end solution. Stability life time is 18 months at 4°C. Our standard tablets are available with a content of 2 mg or 10 mg OPD.

AEC (3-amino-9-ethylcarbazole) is used as a chromogen substrate in immunoblotting and immunohistochemistry. AEC produces a rose-red colour during the enzymatic degradation of H2SO4 by horseradish peroxidase. Each tablet contains 5 mg of AEC and dissolves in approx. 5 minutes. 1 tablet is added 1 ml of 96 % alcohol, followed by addition of distilled water to 10 ml. This will give a solution of pH 5.1 + 0.1 buffered with 20 m…

DAB (3,3-diaminobenzidine tetrahydrochloride), is used as a chromogen substrate in immunoblotting.DAB produces a brown colour during the enzymatic degradation of H2SO4 by horseradish peroxidase. Each tablet contains 10 mg of DAB and dissolves in distilled water in less than 5 minutes. Stability life time is 18 months at 4°C.

Fast Red is used as a chromogen substrate in immunoblotting and immunohistochemistry using alkaline phosphatase antibody conjugates. Each tablet contains 0.5 mg naphthol phosphate, 2 mg FAST RED chromogen and 0.4 mg levamisole, an inhibitor of endogenous enzyme activity. Stability life time is 18 months at 4°C.

The pNPP (p-NitroPhenyl Phosphate) liquid, ready-to-use substrate is a highly active and stable formulation utilized for measuring AP- (Alkaline Phosphatase) probe activity. The solution is a solvent free buffer solution containing stabilized pNPP using sodium azide as preservative. In kinetic assays p-nitrophenol appears yellow and is measured at 405 /650 nm. If stopped reactions are preferred, add equal amounts of 1 .0 M s…

The liquid BCIP/NBT is the most commonly used substrate for colorimetric determination of Alkaline Phosphatase (AP) activity in membrane tests. We offer an aqueous buffered solution containing NBT (NitroBlue Tetrazolium) and BCIP (Bromo-Chloro-Indolyl Phosphate) forming a blue/purple precipitate at the positive sites. The colour develops when AP catalyses the dephosphorylation of BCIP. The intense blue/purple precipitate is…

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New pNPP ONE Substrate – The pNPP (p-NitroPhenyl Phosphate) liquid, ready-to-use substrate is a highly active and stable formulation utilized for measuring AP- (Alkaline Phosphatase) probe activity. The solution is a solvent free buffer solution containing stabilized pNPP using sodium azide as preservative. In kinetic assays p-nitrophenol appears yellow and is measured at 405 /650 nm. If stopped reactions are preferred, add…

The SNAP i.d. Protein Detection System revolutionizes the quality of your blots every time — in record time! Unlike conventional Western blotting, where diffusion is the primary means of reagent transport, the SNAP i.d. system applies a vacuum to actively drive reagents through the membrane. This novel method allows you to optimize your blotting conditions in record time for maximum results. The SNAP i.d. system minimizes over…

Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…

Provides high sensitivity over a broad detection range at an economical price.

LifeXtend™ conjugate stabilizer/diluent is a proprietary multi-component reagent system that stabilizes antibody-HRP conjugates both at 4°C and at ambient temperature. By using LifeXtend™ HRP stabilizer/diluent in antibody conjugation the HRP conjugate can be stabilized during use and stored as pre-diluted, ready-to-use reagents thus eliminating waste and improving consistency from experiment to experiment. LifeXtend™ shows…

Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…