Cell-Based Assays Products & Reviews

The CHEMICON Cell Invasion Assay is performed in an Invasion Chamber, based on the Boyden chamber principle. Each kit contains 24 inserts; each insert contains an 8 μm pore size polycarbonate membrane coated with a thin layer of polymerized collagen. The collagen layer occludes the membrane pores, blocking non-invasive cells from migrating through. Invasive cells, on the other hand, migrate through the polymerized collagen lay…

The CHEMICON Cell Invasion Assay is performed in an Invasion Chamber, based on the Boyden chamber principle. Each kit contains 24 inserts; each insert contains an 8 mm pore size polycarbonate membrane coated with a thin layer of polymerized avian collagen. The collagen layer occludes the membrane pores, blocking non-invasive cells from migrating through. Invasive cells, on the other hand, migrate through the polymerized collag…

The Chemicon® QCM™ 24-well Invasion Assay does not require cell labeling, scraping, washing or counting. The 24-well insert and homogenous fluorescence detection format allows for large-scale screening and quantitative comparison of multiple samples. The CHEMICON® Cell Invasion Assay is performed in an Invasion Chamber, based on the Boyden chamber principle. Each kit contains 24 inserts; each insert contains an 8 μm pore size…

The CHEMICON QCM™ 96-well Invasion Assay does not require cell labeling, scraping, washing or counting. The 96-well insert and homogenous fluorescence detection format allows for large-scale screening and quantitative comparison of multiple samples. The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 96 sa…

The CHEMICON Cell Invasion Assay is performed in a 96-well invasion plate based on the Boyden chamber principle. This plate contains 96 inserts; each insert contains an 8 μm pore size polycarbonate membrane coated with a thin layer of polymerized collagen. The collagen layer occludes the membrane pores, blocking non-invasive cells from migrating through. Invasive cells, on the other hand, migrate through the polymerized collag…

The CHEMICON QCM™ Haptotaxis Cell Migration Assay - Fibronectin, Colorimetric is ideal for the study of epithelial and fibroblast cell haptotaxis. The quantitative nature of this assay is especially useful for large scale screening of pharmacologic agents. BSA-coated control chambers provide an appropriate migration control. The 8 μm pore size of this assay's Boyden chambers is not appropriate for the study of lymphocyte migra…

The CHEMICON QCM™ Haptotaxis Cell Migration Assay - Collagen 1, Colorimetric is ideal for the study of epithelial and fibroblast cell haptotaxis. The quantitative nature of this assay is especially useful for large scale screening of pharmacologic agents. BSA-coated control chambers provide an appropriate migration control. The 8 μm pore size of this assay's Boyden chambers is not appropriate for the study of lymphocyte migrat…

ECM675 uses purified chicken type I collagen in 6mM acetate buffer pH 7.0. The product is 99.9% pure native atelomeric avian collagen. Approximately 85% type I and 15% type III collagen protein. The material has been specially prepared to maintain its native state as much as possible. The telomeric ends of the collagen I have been enzymatically removed. Since most of the species identity is found within the telomeric ends, our…

The Multidrug Resistance Direct Dye Efflux Assay Kit includes two of the best characterized and most commonly used multidrug resistance ABC transporter substrates, DiOC2(3) and rhodamine 123. The kit enables researchers to directly assess the functional activity of the MDR1, MRP1 and BCRP membrane pumps in living cells under physiologic conditions by directly measuring the relative fluorescence of cell populations that activel…

CHEMICON's Actin Cytoskeleton and Focal Adhesion Staining Kit is a very sensitive immunocytochemical tool that contains fluorescent-labeled phalloidin (TRITC-conjugated phalloidin) to map the local orientation of actin filaments within cell and a mouse monoclonal antibody to Vinculin that is very specific for the staining of focal contacts in cells.

The Amplifluor™ UniPrimer™-fluorescein is a hairpin primer labeled with a fluorescent energy transfer pair (fluorescein and non-fluorescent quencher) and a 3' single-stranded oligonucleotide tail and is provided at a concentration of 5.0 μM (25.0 mL).

The Amplifluor™ SNPs Genotyping System for Assay Development is designed for new users unfamiliar with the technology. This kit contains the fluorescein-, sulforhodamine- and JOE-labeled Amplifluor™ UniPrimers™ as well as control primers and templates.

The Amplifluor™ SNPs HT Genotyping System FAM-SR is designed for users familiar with Amplifluor™ and is for use with fluorescent plate readers.

The Amplifluor™ SNPs HT Genotyping System FAM-JOE is designed for users familiar with Amplifluor™ and is for users of real-time PCR detection instruments.

GST fusion-protein, corresponding to the p21-binding domain (PBD, residues 67-150) of human PAK-1 provided bound to glutathione agarose. Specifically binds to and precipitates Rac-GTP and cdc42-GTP from cell lysates.

GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E.coli and bound to glutathione-agarose. Specifically binds to and precipitates GTP-Rho, but not GDP-Rho from cell lysates.

Recombinant protein A covalently bound to magnetic beads. These beads provide users a rapid, reproducible and efficient reagent for collecting immunocomplexes for chromatin immunoprecipitations (ChIP) assays. Compared with conventional protein A agarose beads, protein A magnetic beads significantly reduce the handling time and mechanical stress on target immunocomplexes.

For use to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-Acetyl-Histone H4, ChIP grade rabbit antiserum.

For use to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-acetyl-Histone H3

A GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E. coli. Provided bound to glutathione-agarose. Purity 90%.

Anti-cdc42 is a mouse monoclonal IgG1 that recognizes cdc42 from human, mouse, rat and dog

This kit contains nonradioactive components for radiolabeling and purifying histone H4 peptide with [3H]-acetyl CoA. The radiolabeled peptide is a suitable substrate for assay of HDAC activity. HeLa nuclear extract, which is rich in histone deacetylase activity, is included as a positive control. The HDAC inhibitor sodium butyrate is provided to demonstrate specificity of the deacetylase activity.

Accessory reagent kit for the analysis of histone methyltransferase activity.

This kit provides the reagents required for the detection of citrulline-containing proteins by Western blot analysis with the anti-Citrulline antibody. Detection is a two-step process in which citrulline-containing proteins immobilized on nitrocellulose or PVDF are formaldehyde-cross linked to the membrane to improve protein retention, followed by modification of citrulline residues with 2,3-butanedione monoxime and antipyrine…

This assay is a simple two-step procedure performed in a microtiter plate. In the first step samples are incubated with the HDAC assay substrate, allowing deacetylation of the fluorometric substrate. Next, Activator Solution releases a fluorophore from the deacetylated substrate or standard.