Protein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
RFP -Trap® - use your fluorescence fusion as tag for biochemical studies Curiously RFP, a widely used labeling tag in cell biology, is rarely used for biochemical analyses. This may be due in part to limited RFP antibody availability and specificity as well as co-eluted heavy and light antibody chains that interfere with subsequent analyses. Superior results in a fraction of the time The ChromoTek RFP-Trap® overcomes these pro…
Fusion of proteins to Glutathione S-Transferase (GST) of Schistosoma japonicum is a common technique to increase solubility and expression level of a protein. Furthermore, GST-tagged fusion proteins are often used in protein-protein interaction studies. Both approaches require highly specific tools to isolate and detect GST fusion proteins. GST-Trap The GST-Trap is a highly specific and robust GST-binding protein coupled to ma…
DNA methylation was the first epigenetic modification identified and has been intensively studied for more than half a century. By now it is clear that Dnmt1, the major eukaryotic DNA methyltransferase, faithfully maintains genome-wide methylation patterns and plays an essential role in the epigenetic network controlling gene expression and genome stability during development. However, the molecular mechanisms that ultimately…
Proliferating cell nuclear antigen (PCNA) is a homotrimeric ring-shaped protein that encircles DNA and acts as a loading platform for multiple, transiently interacting factors participating in various DNA transactions. PCNA plays a key role in DNA replication, repair, cell cycle regulation, and post-replicative transactions like DNA methylation and chromatin remodeling. All these cellular processes are regulated by a complex n…
cOmplete His-Tag Purification Resin is an innovative high-capacity IMAC (Immobilized Metal Affinity Chromatography) matrix for convenient single-step purification of His-tagged proteins from lysates. Roche's new proprietary nickel-chelate chemistry is compatible with commonly used reducing agents (such as DTT), chelating metalloprotease inhibitors (such as EDTA), and a wide range of buffer substances and salt conditions. The w…
Introducing an all-in-one centrifugal device for the protein purification workflow.Traditional protein purification is a long process with many steps and multiple devices, often resulting in protein degradation and loss. Avoid the risks associated with sample transfer and reduce hands-on time when you bind, wash, elute and/or concentrate your protein in the all-in-one Amicon® Pro purification system. No matter what your workfl…
The PureEpi Chromatin Preparation and Optimization kit provides a complete set of reagents allowing the preparation of multiple chromatin samples from starting materials such as cultured cells or tissues. A detailed protocol is provided that includes examples of the many variables involved in making chromatin on multiple instruments and how they may affect ChIP results. Features & Benefits • Complete set of materials for up to…
cOmplete His-Tag Purification Column - Prepacked columns with EDTA-compatible resin for the purification of poly-histidine-tagged proteins. cOmplete His-Tag Purification Columns are available in 2 different sizes and prepacked with cOmplete His-Tag Purification Resin. cOmplete His-Tag Purification Resin is an innovative high-capacity IMAC matrix (Immobilized Metal Affinity Chromatography) for convenient single-step purificati…
The PureProteome Protein G Magnetic Bead System is a powerful system that helps researchers purify proteins by maximizing recovery and eliminating variability. PureProteome Protein G magnetic beads are a powerful system to isolate antibodies faster, easier, and with more reproducibility then ever before. PureProteome Protein G magnetic beads purify your sample with the highest binding capacity of any magnetic beads available.…
Purification Consumables and Custom Products.We understand that your purification challenges are unique. That’s why we offer you a variety of options to help you solve them. Whether you seek the up-front cost savings and versatility of packing your own columns, the consistent quality and time savings of pre-packed columns, or the ease associated with having us purify material for you; Sigma-Aldrich is your purification partner…
For homogeneous and Large Volume separations. Production departments need an efficient and economical process to use batches of several liters, with throughputs high enough to meet demand and avoiding particle losses. Sepmag Q is a biomagnetic separation technology specifically developed to process large batch volumes in nucleic acid separation, protein purification, cell isolation, biocatalysts, therapeutics and diagnostics.…
The use of paramagnetic bead based separation devices (MSDs) are an essential accessory in any magnetic bead based purification process. However, traditionally MSDs are not optimized for manual use and most require electrically powered liquid handling systems. The IMAG MSDs in its tube or microplates formats are designed to simplify the manual processing of magnetic bead based separation applications including: Nucleic acid pu…
The use of paramagnetic bead based separation devices (MSDs) are an essential accessory in any magnetic bead based purification process. However, traditionally MSDs are not optimized for manual use and most require electrically powered liquid handling systems. The IMAG MSDs in its tube or microplates formats are designed to simplify the manual processing of magnetic bead based separation applications including: Nucleic acid pu…
1 ml, suspension of protein A conjugated magnetic immunoprecipitation beads (10 mg beads/ml)
3 ml, suspension of protein A conjugated magnetic immunoprecipitation beads (10 mg beads/ml)
1 ml, suspension of protein G conjugated magnetic immunoprecipitation beads (10 mg beads/ml)
3 ml, suspension of protein G conjugated magnetic immunoprecipitation beads (10 mg beads/ml)
Routinely achieve reproducible, accurate, and sensitive protein quantification with ProteinWorks, Waters new family of protein bioanalysis sample preparation kits. ProteinWorks eXpress Digest Kits simplify and accelerate protein digestion, streamlining and standardizing the traditionally complex pre-analytic workflow for LC-MS protein quantification via the surrogate peptide approach. The ProteinWorks uElution SPE Kit…
Mass Spectrometry MS grade manufactured by Wako Pure Chemicals
The EXTRACTMAN ® Starter Kit allows you to be extracting in just minutes.
Asensitive, high-throughput method for quantifying global changes in the level of specific histone modifications
Asensitive, high-throughput method for quantifying global changes in the level of specific histone modifications
Purification of core histones (H2A, H2B, H3 and H4) using very small amounts (as little as 0.9 x 105 cells) of starting material
FG beads are magnetic nanoparticles, suitable for various applications. There are many kinds, which have different functional groups and pre-immobilized proteins.
FG beads® are beads approximately 0.2 μm in diameter that are composed of multiple ferrite particles coated with a special polymer called poly-GMA (glycidyl methacrylate). The FG beads® that are manufactured using this original technology are used as matrices for affinity purification and provide characteristics that are superior to conventional matrices.
