Protein Purification Products & Reviews

Protein A covalently coupled to highly cross-linked agarose beads

This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP's, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release® is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been…

Montage Antibody Purification Kits are designed for fast and easy antibody purification from serum, ascites or cell culture supernatants. The kits include spin columns pre-packed with high capacity PROSEP-A or G media. There are no pumps to setup. No columns to pack. All you need is a centrifuge to purify 10–20 mg in less than 60 minutes.The protocol is easy to follow and, unlike traditional gravity flow methods, the use of ce…

Automation compatible filter units from EMD Millipore are specially designed to provide efficient filtration for dissolution and other automated sample preparation applications.Choice of Filter Media: Automation compatible filter units are available in a variety of membrane types and pore sizes, including units with prefilters for particulate-laden samples.Automation Compatible Packaging: The 200 pack comes preassembled in 8 t…

Millex filter units with hydrophobic Fluoropore membrane are ideal for sterilizing gases, venting sterile containers, and sterilizing or clarifying organic solutions. Specialized filter units protect hemodialysis transducers from blood and moisture. 50 mm Millex filter units are also used for vacuum line protection. All units are bi-directional

Sample preparation for ion chromatography

Filter particle-laden or viscous samples

This 13 mm syringe filter with a Hydrophilic Polyethersulfone membrane, for non-sterile filtration, has a 0.45 micron pore size used in preparation of aqueous & mild organic solutions prior to chromatography or other instrument analyses.

Millex-GP Filter for Samplicity G2, 0.22 µm, PES 33 mm, non-sterile

The PickPen 8-M magnetic tool offers the same benefits as the PickPen 1-M but allows parallel processing of 8 samples. The device is suitable for use with QuickPick™ kits in microplate applications.

The magnetic bead separation device Magnetic PlateX can be used with a Xiril pipetting workstation for automated nucleic acid purification based on the magnetic beads technology. Speed up the process of nucleic acid purification with a magnetic beads kit using the Magnetic PlateX on a Xiril robotic workstation. All pipetting steps are taken over by the robot and the plate gripper “Plate GripX” automatically carries out the tra…

Superior Bead Recovery and Low Levels of False Positives Ensure Assay Reproducibility. Smooth well wall provides efficient bead recovery, ensuring reproducible results lot after lot High performance membrane does not trap microspheres in the membrane matrix In serological assays, Supor® membrane effectively removes IgG complexes, thus reducing nonspecific reactivity of the microspheres and reducing false positives New well des…

Robust Chemical Resistance Provides Compatibility with Harsh Organics. Chemically resistant materials provide a stable platform to process samples in organic solvents Inert materials of construction ensure complete recovery of samples and low non-specific binding New well design results in faster, more uniform filtration rates across the plate and reduces hold-up volume Innovative outlet tip geometry provides direct flow of sa…

QuickPick™ GST kit for proteins, 48 preps (62411)The ready-to-use QuickPick GST glutathione affinity kit is intended for the purification of GST (glutathione-S-transferase)-tagged proteins. It is optimized for use with the PickPen 1-M magnetic tool, and at least 40 ug tagged protein can be purified per prep.

BugBuster® Master Mix combines BugBuster Protein Extraction Reagent with Benzonase® Nuclease and rLysozyme™ Solution in one convenient reagent. BugBuster Master Mix allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. With the Master Mix, there is no need for dilution or separate addition steps. The two available package sizes provide sufficient reagents for protein extracti…

AVOID FREEZE/THAW CYCLES. The ProteoExtract Subcellular Proteome Extraction Kit is a tool for the differential extraction of proteins from adherent or suspension tissue culture cells according to their subcellular localization. The sequential extraction steps yield fractions containing cytosolic proteins, plasma membrane and organelle proteins, soluble nuclear proteins, and finally cytoskeletal and nuclear matrix proteins. To…

PhosphoSafe™ Extraction Buffer extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. The reagent, an enhancement of the CytoBuster™ Protein Extraction reagent, includes four phosphatase inhibitors. PhosphoSafe is compatible with Western blotting, immunoprecipitations, and kinase assays.  

Purification of His•Tag fusion proteins by metal chelation chromatographyThe His•Bind® family of products offers a wide selection of supports designed for rapid one-step purification of proteins containing the His•Tag® sequence by immobilized metal affinity chromatography (IMAC). The His•Tag sequence (6, 8 or 10 consecutive histidine residues) binds to divalent cations (Ni2+) immobilized on NTA*- and IDA-based His•Bind resins.…

miVac is a range of centrifugal vacuum concentrators and freeze driers capable of removing water and organic solvents from a variety of sample formats including tubes, microplates, and vials. miVac concentrators come in 2 sizes, the smaller Duo and a larger Quattro. There is a choice of vacuum pump and rotor according to application and sample format.  To speed concentration built-in special runs for working with water and…

RFP -Trap® - use your fluorescence fusion as tag for biochemical studies Curiously RFP, a widely used labeling tag in cell biology, is rarely used for biochemical analyses. This may be due in part to limited RFP antibody availability and specificity as well as co-eluted heavy and light antibody chains that interfere with subsequent analyses. Superior results in a fraction of the time The ChromoTek RFP-Trap® overcomes these pro…

Fusion of proteins to Glutathione S-Transferase (GST) of Schistosoma japonicum is a common technique to increase solubility and expression level of a protein. Furthermore, GST-tagged fusion proteins are often used in protein-protein interaction studies. Both approaches require highly specific tools to isolate and detect GST fusion proteins. GST-Trap The GST-Trap is a highly specific and robust GST-binding protein coupled to ma…

DNA methylation was the first epigenetic modification identified and has been intensively studied for more than half a century. By now it is clear that Dnmt1, the major eukaryotic DNA methyltransferase, faithfully maintains genome-wide methylation patterns and plays an essential role in the epigenetic network controlling gene expression and genome stability during development. However, the molecular mechanisms that ultimately…

Proliferating cell nuclear antigen (PCNA) is a homotrimeric ring-shaped protein that encircles DNA and acts as a loading platform for multiple, transiently interacting factors participating in various DNA transactions. PCNA plays a key role in DNA replication, repair, cell cycle regulation, and post-replicative transactions like DNA methylation and chromatin remodeling. All these cellular processes are regulated by a complex n…

cOmplete His-Tag Purification Resin is an innovative high-capacity IMAC (Immobilized Metal Affinity Chromatography) matrix for convenient single-step purification of His-tagged proteins from lysates. Roche's new proprietary nickel-chelate chemistry is compatible with commonly used reducing agents (such as DTT), chelating metalloprotease inhibitors (such as EDTA), and a wide range of buffer substances and salt conditions. The w…