UHPLC and HPLC Products & Reviews

ACQUITY UPLC® BEH C18 and C8 columns were designed to be the universal columns of choice for most UPLC® separations by providing the widest pH range.They incorporate trifunctional ligand bonding chemistries which produce superior low pH stability and ultra-low column bleed. This low pH stability is combined with the high pH stability of the 1.7 µm BEH particle to deliver the widest usable pH operating range. In addition, these…

ACQUITY UPLC® BEH Shield RP18 columns combine Waters patented Shield Technology* with BEH Technology™ by incorporating an embedded carbamate group into the bonded phase ligand.Waters shield ligands consistently demonstrate increased retention of phenolic compounds versus straight chain alkyl columns. The alternate selectivity and excellent peak shape from the embedded polar group ligand, when combined with the wide pH range an…

ACQUITY UPLC® BEH Phenyl columns utilize a trifunctional C6 alkyl tether between the phenyl ring and the silyl functionality. This ligand, combined with the same proprietary endcapping processes as the ACQUITY UPLC™ BEH C18 and C8 columns, provides ultra-low column bleed, long column lifetimes and excellent peak shape. This unique combination of ligand and endcap on the 1.7 μm BEH particle creates a new dimension in selectivi…

ACQUITY UPLC® BEH HILIC columns contain the rugged 1.7 μm unbonded BEH particles and are designed to retain and separate very polar basic compounds.These unique columns are optimized and tested to produce efficient and reproducible separations under UPLC® HILIC conditions. ACQUITY UPLC BEH HILIC columns overcome a major weakness of HILIC stationary phases: chemical stability. The unbonded silica stationary phases often used in…

The Waters IdentityE High Definition Proteomics system defines the new standard for rigorous protein identification. The IdentityE High Definition Proteomics System is an UltraPerformance LC® (UPLC)/High-Bandwidth Mass Spectrometry (MS E) system solution suitable for confident protein identification designed to meet stringent requirements as outlined by leading scientific journals.The new Waters IdentityE High Definition Pro…

Run assays up to 5 times faster than a conventional HPLC. Increase mass spec sample throughput by a factor of 5. Eliminate dead time in your analysis and cut solvent usage by 99 percent. Achieve all this and more with the ExpressLC-100 system. TheExpressLC-100 system is a single-channel HPLC with binary solvent delivery, high pressure gradient mixing, variable volume injection, UV absorbance detection, and PC-based instrument…

The ACQUITY UPLC® Fluorescence (FLR) Detector brings unrivaled sensitivity and selectivity to UPLC®. The detector’s innovative flow-cell design, low-noise electronics and support for high-speed data rates are perfectly suited to the efficient separations of the ACQUITY UPLC's advanced fluidics technology. Target applications include PAHs, environmental contaminants, vitamins, aflatoxins and pharmaceuticals. Ultra Performance i…

Column-to-column and batch-to batch reproducibility

SymmetryShield™ Columns feature Waters' patented embedded polar group technology that shields the silica's residual silanols from highly basic analytes at improves overall peak shape. Additionally, by placing the embedded polar group close to the silica surface, the activity of the surface silanols is further reduced. This imparts selectivity and retention that is different compared to the Symmetry C18 ligand. Embedded polar…

Reversed-Phase Protein/Peptide Columns

Over 50 years of reproducible performance

Semi-preparative Columns with excellent batch-to-batch reproducibility

The popular Bondapak® C18 chemistry and Porasil® silica packing material are available in three different particle sizes, 10, 15-20, 37-55 micron, providing easy transfer of chromatography methodology from one particle size to another. You can utilize your existing 10 micron Bondapak or Porasil chromatography as a starting point for scale-up or, if you are developing a new purification protocol, investigate which preparative…

These columns provide accurate and precise molecular weight calculations in under 7 minutes! - Fast, accurate and precise molecular weight determinations - Increase sample throughput - Greatly decrease solvent consumption and disposal

Designed specifically for polymer characterization, the Styragel® columns are grouped into the HR series for low-to-mid molecular weight samples, the HT series for high-temperature applications, and the HMW series for high molecular weight samples. Specifically controlled styrene divinylbenzene formulations provide reproducible performance in your GPC applications.

The Envirogel high-efficiency GPC cleanup columns are specifically designed to remove low volatility, high molecular weight interferences, such as lipids and natural resins, from environmental samples as specified in EPA Method 3640A. In the past, the cleanup procedure for environmental samples was performed on low-efficiency GPC columns based on packing particle diameters of 37-75 micron (200 to 400 mesh) Bio-Beads S-X resins…

For applications where the sample is neither volume or concentration limited, Waters offers 1 and 2mm Internal Diameter columns of various lengths for use on traditional HPLC MS inlets.As with Waters array of NanoEase™ columns, the same Waters reversed-phase packings are also available in these two column internal diameter offerings. Chemistry choices include: XBridge® Columns, CORTECS® Columns, XSelect Columns, Atlantis® Pola…

MassPREP™ On-Line Desalting Cartridges are an effective tool for the successful desalting of acidic, basic, and large globular proteins. The overall MassPREP™ desalting strategy provides an easy, fast (< 3 minutes), and reproducible approach for the effective desalting of proteins stored in physiological salt buffers prior to high resolution ESI-MS structural analysis.

The polar organic and the polar ionic modes have become increasingly popular largely because of their ideal compatibility with MS detection and preparative LC. Two years of extensive research at Astec in understanding these mechanisms has resulted in new possibilities for extending their effectiveness even further. Important changes in linkage chemistries and the silica and the silica have now led to the new CHIROBIOTIC V2 and…

Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we h…

The SPECTRUM range of High Performance CCC (HPCCC) equipment is based on traditional liquid/liquid partitioning and extends the purification options available to chemists, in Discovery and Development, who are looking purify samples from mgs to 5-10 grams of material. The equipment is easily connected and interfaced into existing LC equipment and provides options when existing solid phase technology cannot obtain the resolutio…

The DE Midi range of High Performance CCC (HPCCC) equipment is based on traditional liquid/liquid partitioning and extends the purification options available to chemists, primarily in Development, who are looking purify samples from 5 grams to 1,000 grams of material. The equipment is easily connected and interfaced into existing LC equipment and provides options when existing solid phase technology cannot obtain the resolutio…

Scaling up is easier when using an HPLC media that provides near identical performance across all particle sizes and with increases in column diameter. The separation characteristics of each Phenomenex media offer unique selectivity for each type of application and significant production gains for maximum loadability and process economy. Achieve identical performance from analytical development to large process scale. Our Luna…

SecurityGuard offers a convenient and economical way to extend HPLC column lifetimes. Did you know a common cause of high backpressure, split peaks, broad peaks, baseline noise, baseline drift and loss of resolution is contaminants? The fact is all mobile phases contain some chemical contaminants or microparticulates, from the sample, solvent, or wear on the polymeric seals of the pump or injector. These contaminants can…

SCAT solvent safety systems have been designed to bring solvent safety to the laboratory. The specially designed caps for both solvent reservoirs and waste containers fit virtually any sized container. Certified by TUV in Germany these safety systems af guaranteed to protect your health in the laboratory. Recent legislation has meant the use of solvent safety systems are mandatory in a number of EU countries and several EU m…