High-Throughput Screening > HTS Assay Kits Products & Reviews

A rapid and sensitive homogeneous assay for cortisol assessmentThe HTRF® cortisol assay can be used for the quantification of cortisol in rodent serum, or directly on cells. This assay has also been validated for 11-beta HSD1 screening on whole cells or microsomes.The assay requires only 2 reagent additions, followed by an incubation period of 2 hours at room temperature. It has a detection limit of 70 pg/mL (e.g. 2 fmol/well…

A straightforward tool for biomolecular labeling with Europium cryptateThe Cryptate labeling kit includes all the necessary materials to allow rapid Cryptate labeling of various biomolecules and purification. The labeling reagent Eu3+-TBP-NHS Cryptate is suitable for labeling peptides, proteins, and oligonucleotides, as well as small molecules containing amino groups (N-terminus or lysine residues). The labeling procedure requ…

A range of HTRF® conjugates for assay developmentHTRF®  toolbox assembles a panel of detecting reagents such as Streptavidin-XLlent!, anti-tag antibodies like Eu-Cryptate anti-GST, 6HIS, DNP and anti species conjugated antibodies offering many possibilities for the study of molecular mechanisms. All reagents are available as Cryptate-Europium or XL665 (the two HTRF tracers) conjugates, to allow easy assay development. These re…

The assay involves two monoclonal antibodies, one labeled with Cryptate and the other with XL665, that recognize distinct determinants. The assay is performed in a single step and requires only a short incubation period. Detection limit of the assay is 0.20 ng/mL and the linear range covers 0.3 to 10 ng/mL. The assay has been validated on various biological material including isolated perfused pancreas, plasma, and pancreatic…

Streamlined single plate protocols including all stimulation and cytokine quantification.The HTRFâ cytokine kit line includes TNFa, IFNg, IL1b, IL6 and IL8 assays. These assays feature no separation steps, a high sensitivity and dynamics, and no use of isotopes. Procedures are performed on a single plate with simultaneous cell stimulation and cytokine detection. They can all be run under a similar experimental procedure stream…

A new secondary messenger for Gq coupled receptor investigation.Cisbio’s IP-One assay, based on its proprietary HTRF® technology, is the first HTS immunoassay that can easily detect inositol(1)phosphate (IP1), a stable second messenger which tightly correlates with Gq-coupled receptor activity. This assay displays all the characteristic advantages of an HTRF® system: totally homogeneous, detection via fluorescence, signal stab…

Apoptosis is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis. The apoptotic program is characterized by certain morphologic features, including loss of plasma membrane asymmetry and attachment, condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA. Loss of plasma membrane is one of the earliest features. In apoptotic cells, the mem…

The Amnis NFkB translocation kit includes the conjugated antibody Anti-Hu NFkB (p50) Alexa Fluor 488, the nuclear stain 7-AAD along with the fixation solution, assay buffer and permeabilization buffer to rapidly detect and quantitate NFkB's translocation from the cytoplasm to the nucleus. Nuclear Factor kappa B (NFκB) transcription factor plays a central role in regulating many key processes in mammalian cells, including diff…

Ready-to-use, far-red fixed cell counterstain

Green Flourescent Caspase-3/7 Apoptosis assay for live cell assays. Analysis by flow cytometry, microscopy or fluorescence microplate

Red Flourescent Pan Caspase Apoptosis assay for cell based assays

Green Flourescent Caspase-3/7 Apoptosis assay for live cell assays. Analysis by flow cytometry, microscopy or fluorescence microplate

Green Flourescent Poly Caspase Cell death assay for cell based assays

Green Flourescent Pan Caspase Apoptosis assay for live cell assays

Green Flourescent Caspase 9 Apoptosis assay for cell based assays

His-Tag specific labeling for MST using RED-tris-NTA

The AptaFluor® SAH Methyltransferase Assay Kit enables ultra-sensitive, direct detection of SAH—the universal product of methyltransferase reactions—using a riboswitch-based TR-FRET format. With no coupling enzymes required, it delivers high signal-to-background, Z’ ≥ 0.7, and is fully HTS-compatible for drug discovery and inhibitor screening.

The Luc-Pair™ Duo-Luciferase Assay Kit 2.0 has been improved upon from the Luc-Pair™ Duo-Luciferase Assay Kit. With enhanced stability, it provides a more flexible system in the same convenient 96-well plate format as before for measuring firefly and Renilla luciferases sequentially. Due to its enhanced stability, the Luc-Pair™ Duo-Luciferase Assay Kit 2.0 is the ideal solution for measuring the inhibitory effect of a miRNA on…

Cyto-Cellect™ Human IgGK Detection Kit offers a sensitive and robust assay for the detection of human IgG antibody secretion, ideal for streamlining cell isolation and titer determination.

ClinMax Cytokine Detection Kits

Outstanding GMP QC Testing Services offering Bioassays throughout the lifecycle of drug development, to support the release, stability, and in-process testing of your drug.

For researchers who want to do cell cycle research at their benchtop, Guava Cell Cycle assay can be used for measuring G0/G1, S, and G2/M phase distributions. Based on the established method of whole-cell staining with propidium iodide (PI), Guava's cell cycle assay is easy to learn. Guava Cell Cycle software includes markers that can be set for quick assessment of G0/G1, S, and G2/M phase cell cycle percentages as data is bei…

Apoptosis, or programmed cell death, is a highly regulated pathway that is important in normal developmental processes as well as many diseases. Cells undergoing apoptosis are identifiable by a number of characteristics, including changes in mitochonrial membrane potential, transport of phosphatidylserine (PS) to the membrane surface, activation of caspase proteases, and DNA fragmentation in the nucleus. Guava offers four apop…

Universal Assay for Serine/Threonine KinasesCisbio's line of HTRF® KinEASE™ STK kits are based on our patented HTRF® technology and can be used as a universal tool for assessing Serine/Threonine kinase activity. These assays use a proprietary antibody and a choice of three biotinylated substrates developed by Upstate to rapidly produce high quality kinase results. Our HTRF KinEASE™ STK kits have been validated on more than 107…

Cisbio's HTRF® KinEASE™ platform has expanded to include a universal assay for tyrosine kinases. The HTRF KinEASE TK assay is based on our patented HTRF technology and uses a unique tyrosine peptide substrate in combination with a Europium-labeled monoclonal antibody. The HTRF KinEASE™ TK assay has been validated on 59 tyrosine kinases to-date including both receptor and cytoplasmic TKs. A proprietary substrate stabilizing buf…