Isolate total RNA from formalin-fixed, paraffin-embedded tissue as well as fresh-frozen, tissue-research samples for use in RT-PCR with the High Pure RNA Paraffin Kit. The quality of RNA obtained with this kit is suitable for relative quantification of mRNA with RT-PCR, especially with the LightCycler® Carousel-Based System. Benefits Can isolate RNA suitable for RT-PCR from archived paraffin tissue (up to 15 years old). Yi…
Use the High Pure 96 UF Cleanup Kit to purify amplification products for direct use in applications such as microarray spotting or sequencing. The rapid protocol allows simultaneous purification of 96 reactions in less than 20 minutes with excellent recovery. Benefits Choose a flexible format. Samples can be processed manually using vacuum manifolds or microplate centrifuges, or using an automated method on standard liquid-…
The kit is designed for the efficient and convenient isolation of PCR products from amplification reactions. Primers, mineral oil, salts, unincorporated nucleotides, and the thermostable DNA polymerase may inhibit subsequent enzymatic reactions (e.g., labeling, sequencing or cloning of the PCR products). In addition, nucleic acids from other modification reactions (e.g., restriction-endonuclease digests, alkaline-phosphatase t…
The High Pure Viral RNA Kit is intended for general laboratory use and is designed to purify viral RNA from serum or plasma. Viral RNA is used for RT-PCR analysis directly after elution in nuclease-free water. Principle As a prerequisite for the reverse transcription polymerase chain reaction (RT-PCR), viral RNA must be isolated from serum or plasma. Virus lysis is accomplished by incubation of the sample in a special Lysis/Bi…
The High Pure Nucleic Acid Kit is intended for general laboratory use and is designed to purify viral nucleic acids from serum, plasma, or whole blood, using sample volumes of up to 200 µl. When using whole blood, total nucleic acids are isolated, including viral nucleic acids. The purified viral nucleic acid is used for PCR and RT-PCR analysis directly after elution in nuclease-free water. PrincipleAs a prerequisite for PCR o…
This kit is used to prepare purified plasmid DNA in small quantities, known as “mini-preps”, using the alkaline lysis method, a commonly used method that generates highly purified plasmid DNA from E. coli, free of RNA contamination. The kit is designed for the isolation of up to 10 µg of plasmid DNA from 0.5 to 4 ml of bacterial suspension. Up to 24 samples can be processed at the same time in less than 45 minutes. The purifie…
This kit is designed to purify nucleic acids from different sample materials, including whole blood, cultured cells, and tissue samples. Bacteria and yeast require a specific prelysis treatment with lysozyme or lyticase. The resulting nucleic acids are ready for use in PCR and restriction-digestion reactions. Benefits Maximize flexibility in sample preparation. This versatile kit works well with a wide variety of sample ma…
Use the High Pure FFPE RNA Micro Kit to isolate total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples for direct use in RT-PCR. The isolated RNA is suitable for relative quantification of mRNA in RT-PCR using real-time PCR systems such as the LightCycler® Carousel-Based System and the LightCycler® 480 System. Isolate total RNA from 1 to 10 µm sections of formalin-fixed, paraffin-embedded tissue (e.g., colon, b…
The Genopure Plasmid Midi Kit is used to prepare purified plasmid DNA in medium quantities known as “midi preps”. Using a modified alkaline lysis method, highly purified plasmid DNA from E. coli (free of RNA contamination) is generated. The kit is designed for the isolation of up to 100 µg of plasmid DNA from bacterial culture. Depending on the number of plasmids, use a 5-ml to 30-ml (high-copy number) or 10-ml to 100-ml (low-…
The Fast-Trap Lentivirus Purification and Concentration Kit contains the reagents, filtration devices, and concentration devices necessary to purify the virus away from cellular contaminants and the expressed recombinant transgene. It yields concentrated virus in the exchange buffer of choice, suitable for in vitro and animal studies. The kit provides a quick, easy, membrane-based method for the laboratory scale purification o…
The Fast-Trap Adenovirus Purification and Concentration Kit contains the reagents, filtration devices, and concentration devices necessary to purify the virus away from cellular contaminants and the expressed recombinant transgene. It yields concentrated virus in the exchange buffer of choice, suitable for in vitro and animal studies. The kit provides a quick, easy, membrane-based method for the laboratory scale purification o…
For purification of up to 20 µg molecular biology grade plasmid DNA
Built on Solid Phase Reversible Immobilization (SPRI) paramagnetic bead-based technology, the Agencourt DNAdvance kit is a high throughput genomic DNA (gDNA) isolation reagent kit enabling the purification of high quality DNA from mammalian tissue samples.
The Empore™ High Performance Extraction Disk Plate differs in design from plates offered by other manufacturers and requires a specific vacuum manifold. Manifolds supplied by different plate manufacturers are specific to the design of their plates and cannot generally be used with plates from other manufacturers. In some cases, the use of spacers to raise/lower the height of a collection device inside the manifold may overco…
Replacement gaskets are available with the vacuum manifold assembly. The gasket can become worn over time and the seal between the plate and the manifold will be affected. This may result in poor vacuum pressure and negatively effect flow rates. The gasket should be checked regularly and replaced when it becomes worn.
The AxyPrep™-96 Plasmid Purification Kit represents a true high-throughput system for the purification of plasmid or cosmid DNA in a 96-well format. The AxyPrep™-96 Plasmid Purification Kit is designed to produce up to 20 mg of highly purified plasmid or cosmid DNA (per well) from up to 1.3 ml of bacterial culture in 96-well format. With the AxyPrep™-96 Plasmid Purification Kit two plates rapidly and efficiently process the…
AxyPrep™ Easy-96 Plasmid Purification Kit employs a modified alkaline lysis method in combination with an optimized 96-well lysate filtration plate and an alcohol precipitation step to purify plasmid and cosmid DNA from bacterial cultures. The AxyPrep™ Easy-96 Plasmid Purification Kit is designed to process 1.3 ml aliquots of bacterial culture, grown in a 96-well format. Plasmid or cosmid DNA prepared by the Axyprep Easy-96…
This kit is based upon a modified SDS-alkaline lysis of bacterial cells in combination with lysate filtration and selective binding of the plasmid DNA to special AxyPrep purification columns. The yield of plasmid for this kit is up to 100 mg. This kits can also be used to isolate large constructs, such as BAC, PAC and P1. The protocols provide a simple, rapid and efficient method for the isolation of highly purified plasmid…
This kit is based upon a modified SDS-alkaline lysis of bacterial cells in combination with lysate filtration and selective binding of the plasmid DNA to special AxyPrep purification columns. The yield of plasmid for kit is up to 500 mg respectively. This kit can also be used to isolate large constructs, such as BAC, PAC and P1. The protocols provide a simple, rapid and efficient method for the isolation of highly purified p…
AxyPrep™ DNA Gel Extraction Kit employs optimized reagents in combination with a convenient spin column to purify DNA fragments from either TAE or TBE agarose gels (regular and low-melt). Each AxyPrep column in the AxyPrep DNA Gel Extraction Kit will bind up to 8 µg of DNA. DNA fragments in a size range of 75 bp up to 10 kb can be efficiently recovered with the AxyPrep DNA Gel Extraction Kit. Depending upon the length of the…
AxyPrep Blood Genomic DNA Miniprep Kit - Suitable for purifying up to 12 mg of genomic DNA from 250 ml of anti-coagulated whole blood. Blood genomic DNA is isolated directly from the white blood cell (WBC) component of whole blood, without the need to remove the red blood cells (RBCs) in advance. The isolation of genomic DNA from blood using the AxyPrep Miniprep Kit is based on the efficient release of genomic DNA from by a…
This kit provides a rapid and economical method for the purification of plasmid DNA in a convenient mini column format. Each AxyPrep column has a binding capacity of at least 20 mg of plasmid DNA. AxyPrep columns are designed for use in either a spin or vacuum format. Each column can process up to 4 ml of bacterial culture grown in LB or up to 2 ml of culture grown in rich broth. The entire procedure can be completed within…
The AxyPrep™ Blood Genomic DNA Midiprep kits are designed to produce up to 100 mg and 500 mg of high molecular weight genomic DNA respectively from anticoagulated human or animal blood. Purification with the AxyPrep Blood Genomic DNA Midiprep Kit is based upon a unique phase-partitioning technology in combination with the selective binding of the genomic DNA to a special AxyPrep column. The purified genomic DNA is greater th…
The AxyPrep™ Blood Genomic DNA Maxiprep kits are designed to produce up to 100 mg and 500 mg of high molecular weight genomic DNA respectively from anticoagulated human or animal blood. DNA Purification with the AxyPrep Blod Genomic DNA Maxi Prep Kit is based upon a unique phase-partitioning technology in combination with the selective binding of the genomic DNA to a special AxyPrep column. The purified genomic DNA is greate…
AxyPrep™ Body Fluid Viral DNA/RNA Miniprep Kit provides a rapid and highly reproducible way to purify viral nucleic acid from 250 ml of body fluid, including plasma, serum, ascites, cerebrospinal fluid, urine, cell culture supernatant, etc. With the AxyPrep™ Miniprep Kit, during lysis, proteins and PCR inhibitors are removed by denaturation and precipitation. Viral nucleic acids remain soluble in the supernatant and are puri…
