Western Blotting Products & Reviews

The Quick Western Kit provides a universal detection reagent that can be combined with the primary antibody incubation step, eliminating the need for a secondary antibody incubation step.

IRDye 680RD is the near-infrared fluorescent dye of choice for applications in the 700 nm wavelength, especially Western blots, cell-based assays such as the In-Cell Western assay, and small animal imaging experiments.

Odyssey One-Color Protein Molecular Weight Markers fluoresce in the 700nm channel of any of the Odyssey Imaging Systems and contain ten protein bands of 10, 15, 20, 25, 37, 50, 75, 100, 150, and 250 kDa, with molecular weight shifts of 5% and minimal band broadening.

NewBlot Western Blotting Stripping Buffers conserve precious samples by reusing the same blot and you save money.

The LICORbio C-DiGit Chemiluminescence Western Blot Scanner is the perfect integration of quality and price. The C-DiGit Scanner helps you develop film-quality images without the hassle and expense of film development.

IRDye Infrared Dyes and near-infrared (NIR) fluorescent imaging deliver enhanced sensitivity due to low background autofluorescence in the near-infrared region, therefore, giving a higher signal to noise ratios.

IRDye and VRDye secondary antibodies are highly cross-adsorbed, making them suitable for multi-color detection. IRDye secondary antibodies have been optimized for use with the Odyssey® family of imaging systems and can be used on instruments with similar excitation and emission filters.

When performing two-color detection, loading controls and normalization are essential for reliable, precise comparison of protein levels. LICORbio offers primary antibodies for normalization that can be used for two-color normalization when performing multiplex Western blots or for cell-based assay normalization.

CellTag 700 Stain is a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications. The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers.

Chameleon®  Pre-Stained Protein Ladders offer easy visualization of gel migration and protein size, and can be used to orient your gel or membrane quickly. These versatile protein markers offer multi-colored, pre-stained proteins for both visual inspection and two-color near-infrared detection. Choose from either single-channel or two-channel detection.

Visualize and quantify the total protein content on Western blot membranes with Revert 700 Total Protein Stain. Revert 700 Total Protein Stain provides linear, proportional signal across a broad range of sample concentrations.

4X Protein Sample Loading Buffer is optimized for use as a loading buffer for protein gel electrophoresis. This orange loading buffer is recommended for use with Odyssey® Imaging Systems as it does not fluoresce in the 700 channel.

Chameleon Vue Pre-stained Protein Ladder offers multi-colored, pre-stained proteins for molecular weight estimation

Chameleon Duo Pre-stained Protein Ladder provides multi-colored, pre-stained bands for visual inspection and two-color near-infrared detection. It includes 11 protein bands from 8 to 260 kDa.

IRDye Labeling Kits come in 3 forms and allow you to label your own peptides, antibodies, or proteins.

IRDye 800CW secondary antibodies are the antibodies of choice for a wide variety of applications in the 800 channel of Odyssey ® Imaging Systems.

Odyssey Loading Indicators (OLI) provide a simple, convenient method to evaluate the consistency of sample loading volume across gel lanes, as well as the uniformity of Western blot transfer.

The Odyssey Pen is suitable for writing on membranes to be scanned on any of the Odyssey Imagers.

Empiria Studio Software is the ideal software for labs that want workflow-based analysis to ensure consistent results for all researchers. Researchers of all experience levels can easily determine target protein expression, analyze validated antibody experiments, compare replicates, perform statistical analysis, share results, and much more—all in one intuitive application.  

Intercept T20 Antibody Diluents for use with Odyssey® Imaging Systems, are optimized for use with IRDye® Infrared Dye Reagents and other near-infrared fluorophores.

Try Intercept (TBS) Blocking Buffer or Intercept (PBS) Blocking Buffer for improved results in your quantitative Western blots and other immunoassays. Intercept Blocking Buffers provide excellent blocking performance with low background and low variability.  

The Odyssey XF is the perfect entry point for labs transitioning from qualitative chemiluminescent Western blotting to more robust quantitative data analysis. Drawing from more than 30 years of imaging expertise, the Odyssey XF offers unmatched dynamic range with the click of a mouse. In addition to two NIR lasers, it has LED and CCD capabilities, providing multi-functional versatility for greater value.

CellTag 520 Stain is a visible fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications.

The Odyssey Fc System is the only imaging system that offers superior chemiluminescent detection and the benefits of quantitative infrared Western blot detection as well as the ability to document DNA gels using the 600nm channel.

Imaging probes conjugated to IRDye 800CW and IRDye 700DX have propelled more than 20 Phase I and Phase II clinical trials worldwide.