Proteomics Products & Reviews

The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin.

Three different strains that provide varying levels of expression control with T7 promoter-driven vectors, such as the pCAL and pET vectors.BL21 derivatives for tight expression control Express toxic proteins Use BL21, BL21-Gold or BL21-CodonPlus cells High-level protein expression Use for established expression constructs Economical alternative

BL21-CodonPlus strains are engineered to contain extra copies of genes that encode the tRNAs that most frequently limit translation of heterologous proteins in E. coli. They are ideal for difficult protein expression, especially when codon bias is a problem.

The pET E. coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene.

The pBK-CMV vector allows you to express proteins in both E. coli and mammalian model systems and saves you valuable time by not having to subclone into an additional vector.  In mammalian cells, the expression is driven by the CMV promoter and is therefore constitutively expressed.  In E. coli, the lac promoter drives high-level expression in the presence of the inducer IPTG, and allows minimal expression in the absence of i…

The Complete Control Inducible Mammalian Expression System is designed to provide the tightest control of expression available in mammalian cells. The system is based on a synthetic ecdysone-inducible receptor and a synthetic receptor recognition element that modulates expression of your gene of interest.  

The LacSwitch II inducible mammalian expression system utilizes an improved vector system in which several elements of the lac operon have been modified for use in eukaryotic cells for the control of gene expression.

Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study. The pCMV-Tag vectors have a single copy of the available epitope tags, while the pCMV-3Tag vectors have three copies of the epitope tag (in a variety of configurations) to prove a stronger signal in immmunological assays.

Method completed in less than one day versus 2 days with restriction enzymes

The pSG5 Vector is a 4.1 Kb eukaryotic expression vector constructed by combining pKCR2 and the Stratagene pBS vector. The high copy number of this plasmid allows for large quantities of double-stranded DNA to be obtained. Expression in vivo is achieved via transient expression in a variety of cell lines (highest level of expression is obtained following transfection of a cell line expressing the T antigen).

StrataClean resin eliminates the need to perform phenol:chloroform extractions, which are highly toxic and combustible. The resin is a non-toxic, non-combustible slurry of hydroxylated silica particles that is used to clean up DNA or concentrate dilute protein mixtures.

The StrataClone Mammalian Expression Vectors were designed for high-level expression of epitope-tagged proteins in mammalian cells. Use of StrataClone technology for the cloning steps allows rapid cloning of blunt-ended PCR products, produced using high-fidelity PCR enzymes, into the epitope tagging/expression vectors.

Our Mycoplasma Plus PCR Primer Set detects infection by all forms of Mycoplasma with a robust single-band PCR product (triplet for Acholeplasma). This kit also includes controls to verify PCR amplification and Mycoplasma genomic DNA. The primer design permits higher annealing temperatures, reducing spurious bands from PCR contaminants.

The MycoSensor PCR & QPCR Assay Kits detect Mycoplasma infection of cell cultures by real-time quantitative PCR (qPCR) or PCR in less than two hours.

The Mx3005P system is among the most reliable and trusted QPCR instruments available, with a long record of citation in peer-review journals. The Agilent Mx3005P and Mx3000P QPCR Systems are the most flexible—and reliable—instruments available for gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis, and chromatin immuno-precipitation studies.Highly reproducible re…

The GeneMorph II Random Mutagenesis Kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis. Other methods or commercially available kits that rely on Taq DNA polymerase for random mutagenesis have shown biases towards mutating A’s and T’s more frequently than G’s and C’s, which undoubtedly skews representation of random mutant libraries.

Agilent’s Absolutely RNA Product portfolio makes RNA purification from tissue and cell samples easy and fast. Absolutely RNA kits include all the reagents you need for DNA-free total RNA, and miRNA isolation with no additional purchase of DNase required. No heating, no long centrifugation, and no repurification is required. Each absolutely RNA purification kit has been optimized for a specific range of sample sizes and elutio…

The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae.  Each vector contains GAL1 and GAL10 yeast promoters in opposing orientations. With these vectors you can introduce one or two cloned genes into a yeast host strain under the control of an inducible promoter.

Agilent’s Absolutely RNA Product portfolio makes RNA purification from tissue and cell samples easy and fast. Absolutely RNA kits include all the reagents you need for DNA-free total RNA, and miRNA isolation with no additional purchase of DNase required. No heating, no long centrifugation, and no repurification is required. Each absolutely RNA purification kit has been optimized for a specific range of sample sizes and elution…

Agilent’s Absolutely RNA Product portfolio makes RNA purification from tissue and cell samples easy and fast. Absolutely RNA kits include all the reagents you need for DNA-free total RNA, and miRNA isolation with no additional purchase of DNase required. No heating, no long centrifugation, and no repurification is required. Each absolutely RNA purification kit has been optimized for a specific range of sample sizes and elutio…

Agilent’s Absolutely RNA Product portfolio makes RNA purification from tissue and cell samples easy and fast. Absolutely RNA kits include all the reagents you need for DNA-free total RNA, and miRNA isolation with no additional purchase of DNase required. No heating, no long centrifugation, and no repurification is required. Each absolutely RNA purification kit has been optimized for a specific range of sample sizes and elutio…

Agilent’s Absolutely RNA Product portfolio makes RNA purification from tissue and cell samples easy and fast. Absolutely RNA kits include all the reagents you need for DNA-free total RNA, and miRNA isolation with no additional purchase of DNase required. No heating, no long centrifugation, and no repurification is required. Each absolutely RNA purification kit has been optimized for a specific range of sample sizes and elutio…

Agilent’s Absolutely RNA Product portfolio makes RNA purification from tissue and cell samples easy and fast. Absolutely RNA kits include all the reagents you need for DNA-free total RNA, and miRNA isolation with no additional purchase of DNase required. No heating, no long centrifugation, and no repurification is required. Each absolutely RNA purification kit has been optimized for a specific range of sample sizes and elutio…

Agilent’s Absolutely RNA Product portfolio makes RNA purification from tissue and cell samples easy and fast. Absolutely RNA kits include all the reagents you need for DNA-free total RNA, and miRNA isolation with no additional purchase of DNase required. No heating, no long centrifugation, and no repurification is required. Each absolutely RNA purification kit has been optimized for a specific range of sample sizes and elutio…

Agilent’s Absolutely RNA Product portfolio makes RNA purification from tissue and cell samples easy and fast. Absolutely RNA kits include all the reagents you need for DNA-free total RNA, and miRNA isolation with no additional purchase of DNase required. No heating, no long centrifugation, and no repurification is required. Each absolutely RNA purification kit has been optimized for a specific range of sample sizes and elutio…