An arrayed or non-arrayed BAC library is the preferred choice for downstream applications such as hybridization screening; full-genome sequencing; BAC end sequencing or physical mapping projects. Amount of clones received is based on a guaranteed coverage and 120Kb average insert size. HMW DNA isolation and QC by HMW DNA enzymatic digestion are included. Deliverables for ARRAYED BAC library: Clones will be arrayed and amplifie…
A pooled BAC library is an invaluable tool for PCR-screening. It allows for the easy retrieval of BAC clones of interest. This type of library is especially useful when genome size is large and creating an arrayed library is cost-prohibitive. We typically construct a minimum 5X BAC library and BAC clones are then pooled and distributed in 96-well plates. Each well will contain about 400-500 original BAC clones. HMW DNA isolati…
Our cDNA libraries feature full-length-enriched and high quality size-fractionated cDNA. cDNA library construction is usually done in slightly-modified versions of pBluescript or pcDNA3.1. Insert sizes will range from about 0.5Kb to 4Kb. Concerning QC steps, we evaluate the average insert size, empty clone rate and clone concentration/tube before shipping out. We always provide a gel picture of randomly selected clones showing…
APAgene™ GOLD Genome Walking Kits are designed to rapidly and reliably amplify unknown genomic DNA using our patented APA technology: 1. Isolate unknown genomic sequences flanking transgenes (including T-DNAs, gene traps, transposons), sequence-tagged sites (STSs) or expressed sequence tags (ESTs). 2. Isolate localized sequences flanking known sequences from large clones and genomic DNAs. 3. Sequence insert ends of large clone…
ASH is APA-based, full-length-enriched, single-stranded cDNA subtraction hybridization library. - ASH cDNA subtraction is full-length-enriched and independent of PCR suppression. - ASH cloning is directional and does not involve normalization. - ASH method provides non-exponential amplification before subtraction because double-stranded cDNA is not required. - ASH method has a better subtraction effect because 1) a high ratio…
Fosmid clones are similar in size (ca. 40kb) to cosmids, but, like BAC clones, they contain replicons derived from the F factor for DNA replication and segregation. Because of this property, they are present in low copy number and consequently more stable than cosmids. Also, the copy control vector can be used for easy DNA purification from fosmid clones. Fosmid libraries are commonly used for closing gaps in a whole genome se…
Discover More, Sequence Less Built upon the NimbleGen Sequence Capture empirically optimized probe design algorithm with high efficiency uniformity of capture1, and now with expanded coverage of coding exons, SeqCap EZ Exome v3.0 offers increased performance. Capture the most comprehensive picture of the human exome with SeqCap EZ Exome Library v3.0. Based on the latest database builds and offering a 64Mb sequence capture, Seq…
The Thermo Scientific ToxInsight Drug Induced Liver Injury Assay Cartridge for the ToxInsight IVT Platform offers a new multi-parameter cell-based assay to determine the risk of liver injury by chemicals being tested for new drugs or for consumer products safety assessment. The ToxInsight DILI Cartridge is for identifying compound hepatotoxicity by simultaneous detection of multiplexed targets, including cell loss, nuclear DNA…
The Thermo Scientific ToxInsight Endocrine Profiler Panel offers a complete solution for consumer products companies needing to comply with regulatory Reproductive Toxicity testing requirements. Traditional methods of assessing endocrine disruption in vitro measure single targets in homogeneous formats and have a record of low predictive power. The Thermo Scientific ToxInsight Endocrine Profiler Panel leverages our proven tech…
The AD1520™ is a tabletop workstation designed for high speed aspirating and dispensing applications to glass slides, microtiter plates or membranes. The system is compatible with multiple dispensing technologies, has nest options and can be customized to meet application needs making it ideal for a research laboratory to investigate new applications.
Reliability, speed, traceability. Characterised by its ease-of-use, speed and image quality, the InnoScan 710 from Innopsys is an ideal tool for routine microarray reading. Supplied with a validation slide and associated Mapix software, full compatibility with traceability standards is assured.InnoScan 710 has a special feature of using a real-time autofocus system that offers the advantage of making it insensitive to substra…
Performance, Resolution, Speed. With its new detection system, the InnoScan 910 has the highest resolution and reading speed on the market. This user-friendly, two-color scanner is the ideal tool for reading current and future high-density microarrays.With outstanding sensitivity and signal-to-noise ratio derived from its highly regarded photometric properties, the InnoScan 910 is a confocal detection scanner fitted with 2 ex…
Rapidity, automation, performance. Saving time and enhancing convenience the InnoScan AutoLoader can be incorporated into the InnoScan 710 and 910 fluorescence scanners from Innopsys to enable a series of slides to be analysed unattended.The InnoScan AutoLoader is compatible with a wide variety of microarrays (DNA, Protein, aCGH, etc.) from different suppliers and Innopsys’ own microarrays. When combined with the InnoScan ins…
Simple, User-friendly, High performance.Mapix is characterised by its ease-of-use. It is a standard in the field of segmentation (automatic searching for microarray spots). Mapix has the most up to date requirements to browse data from microarray experiments quickly, with a high level of reliability and optimised productivity.Mapix can be customized, allowing users to add their own functions using its exclusive plug-in system.…
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For simultaneous purification of DNA and RNA from cells and tissues in 96-well format
The miScript miRNA PCR Arrays accurately analyze the expression of multiple microRNA (miRNA) sequences at the same time on ANY real-time PCR instrument. This new generation of miRNA PCR Array builds on the original strengths of the RT² miRNA PCR Arrays* with new pathway content, expanded assay coverage plus added species all with the flexibility and sample preparation advantages of the miScript PCR System. The available 96-…
The ultimate standard for autoclaveable bench-top fermentor systems Comes with all necessary controls and accessories for fermentation or cell culture Can operate in continuous, batch, and fed-batch modes Size range: 2.0-7.0 L Option to run up to 6 fermentors at a time from one station with our state-of-the-art BiOSCADA software system: offering countless options for control strategy, process engineering, and data manag…
The in-situ sterilizable fermentor that is small in size, but large in results: Smallest in-situ sterilizable bench-top fermentor on the market Attractive choice in research and educational settings, especially for scale-up and screening purposes Range of options for both culture media mixing and gas dispersion allows for all kinds of aerobic cell cultures, anaerobic cell cultures, and even micro-carrier cultivations. S…
The flexible & mobile laboratory fermentor for cultivation excellence: Designed for laboratory or small-scale production purposes In-situ sterilizable, with electrical heating system or steam heat exchanger As a modular system with glass or steel cylinders, can readily adapt to a wide variety of needs with minimum hassle Can operate in continuous, batch, and fed-batch modes Available as a cGMP fermentor for small-s…
The classically designed, yet never duplicated laboratory fermentor: Universally designed for a wide-range of applications with low operating costs Medium volumes can be produced even if there is no steam available in your lab Consistent design philosophy, the concept has never been modified since its existence although others have certainly tried to replicate it Available in a steel head-glass cylinder or steel head…
The perfect link between laboratory and production: Designed for scale-up or production processes User-friendly and open-frame construction allows for easy installation and maintenance of this in-situ sterilizable fermentor, with steam heat exchanger Modular design gives flexibility when choosing controls and accessories for any biotechnological process. Size range: Choose from an option of 42L, 50L, or 75L stainless…
The pilot-scale fermentor for production perfection: Our largest off-the-shelve fermentor, in-situ sterilizable for use in pilot and production plants Straight-forward and user-friendly design, with flexible controls and accessories for whatever performance you are looking to achieve Size range: Total volume size ranging from 100L to 1000L and possibly beyond Controlled by our state-of-the-art BiOSCADA software: offer…
Flat bottoms with 112 µL total volume Recommended working volumes of 20 to 80 µL TC-treated for optimal cell attachment Nonreversable lids with condensation rings to reduce contamination Sterilized by gamma radiation and certified nonpyrogenic Individual alphanumeric codes for well identification Bulk packed 20 per bag
Flat bottoms Nonreversible lids with condensation rings to reduce contamination Individual alphanumerical codes for well identification Uniform footprint for ease in stacking Treated for optimal cell attachment Sterilized by gamma irradiation Certified nonpyrogenic
