Chemicon's Mast Cell Degranulation Assay Kit provides a simple and convenient means for assaying tryptase activity. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate tosyl-gly-pro-lys-pNA. The free pNA can then be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. A pNA Standard is provided as a comparison control.
CHEMICON's NS220 Neurite Outgrowth Assay Kit (3 µm) utilizes microporous tissue culture insert technology from EMD Millipore and is based on the use of Millicell cell culture inserts (chambers) containing a permeable membrane with 3-μm pores at the base. The Millicell inserts utilized in this kit are appropriate for cells projecting neurites of up to 3 µm diameter, e.g. N1E-115 cells [Abe et al., 2003], Dorsal Root Ganglia and…
CHEMICON's NS225 Neurite Outgrowth Assay Kit (1 μm) utilizes microporous tissue culture insert technology from EMD Millipore and is based on the use of Millicell cell culture inserts (chambers) containing a permeable membrane with 1 μm pores at the base. With this system, it is possible to screen numerous biological and pharmacological agents simultaneously, directly evaluate adhesion and guidance receptor functions responsibl…
ApopTag® Equilibration Buffer is intended for use in labeling the 3'-OH ends of fragmented DNA during apoptotic cell detection. Qualified for use with Apoptag® In Situ Apoptosis Detection Kits
ApopTag® Terminal deoxynucleotidyl Transferase (TdT) is a recombinant enzyme intended for use in labeling the 3'-OH ends of fragmented DNA during apoptotic cell detection. Qualified for use with Apoptag® In Situ Apoptosis Detection Kits
Rat mammary gland undergoes programmed cell death processes during the tissue regression that follows lactation. Apoptotic bodies, which can be seen by routine histologic staining, can be specifically stained by techniques that localize DNA strand breaks in situ. The specificity of staining for strand breaks can thus be correlated with the morphological changes that define the process of apoptosis.
APOPTAG® Plastic Coverslips are designed to be custom fitted to the specific APOPTAG® kit application.
The ApopTag® Peroxidase In Situ Oligo Ligation Kit specifically detects apoptosis by staining only cells that contain double-stranded breaks that are blunt-ended or have a one base 3' overhang (cells containing nicked, gapped, 3'-recessed, 3'-overhanging ends longer than one base and single-stranded ends are not detected). The high analytic specificity of the ApopTag® ISOL Kit minimizes background due to non-specific labeling…
The Amplifluor™ UniPrimer™-fluorescein is a hairpin primer labeled with a fluorescent energy transfer pair (fluorescein and non-fluorescent quencher) and a 3' single-stranded oligonucleotide tail and is provided at a concentration of 5.0 μM (25.0 mL).
The Amplifluorâ„¢ SNPs Genotyping System for Assay Development is designed for new users unfamiliar with the technology. This kit contains the fluorescein-, sulforhodamine- and JOE-labeled Amplifluorâ„¢ UniPrimersâ„¢ as well as control primers and templates.
The Amplifluorâ„¢ SNPs HT Genotyping System FAM-SR is designed for users familiar with Amplifluorâ„¢ and is for use with fluorescent plate readers.
The Amplifluorâ„¢ SNPs HT Genotyping System FAM-JOE is designed for users familiar with Amplifluorâ„¢ and is for users of real-time PCR detection instruments.
CHEMICON's Sure Blot® CHEMI™ Hybridization & Detection Kit is designed to provide high quality, non-radioactive detection of digoxigenin-labeled DNA probes hybridized to Sure Blot® Nylon Membrane. As an integrated hybridization and detection system, Sure Blot® CHEMI™ is quality controlled to produce high signal and low background. Comparable sensitivity to 32P is typically observed for single-copy gene detection. Sufficient re…
GST fusion-protein, corresponding to the p21-binding domain (PBD, residues 67-150) of human PAK-1 provided bound to glutathione agarose. Specifically binds to and precipitates Rac-GTP and cdc42-GTP from cell lysates.
GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E.coli and bound to glutathione-agarose. Specifically binds to and precipitates GTP-Rho, but not GDP-Rho from cell lysates.
Recombinant protein A covalently bound to magnetic beads. These beads provide users a rapid, reproducible and efficient reagent for collecting immunocomplexes for chromatin immunoprecipitations (ChIP) assays. Compared with conventional protein A agarose beads, protein A magnetic beads significantly reduce the handling time and mechanical stress on target immunocomplexes.
For use to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-Acetyl-Histone H4, ChIP grade rabbit antiserum.
For use to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-acetyl-Histone H3
A GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E. coli. Provided bound to glutathione-agarose. Purity 90%.
Anti-cdc42 is a mouse monoclonal IgG1 that recognizes cdc42 from human, mouse, rat and dog
The cocktails in this kit are intended for the inhibition of cellular Serine/Threonine protein phosphatases, Tyrosine phosphastases, acid and alkaline phosphatases.
This kit contains nonradioactive components for radiolabeling and purifying histone H4 peptide with [3H]-acetyl CoA. The radiolabeled peptide is a suitable substrate for assay of HDAC activity. HeLa nuclear extract, which is rich in histone deacetylase activity, is included as a positive control. The HDAC inhibitor sodium butyrate is provided to demonstrate specificity of the deacetylase activity.
Accessory reagent kit for the analysis of histone methyltransferase activity.
This kit provides the reagents required for the detection of citrulline-containing proteins by Western blot analysis with the anti-Citrulline antibody. Detection is a two-step process in which citrulline-containing proteins immobilized on nitrocellulose or PVDF are formaldehyde-cross linked to the membrane to improve protein retention, followed by modification of citrulline residues with 2,3-butanedione monoxime and antipyrine…
This assay is a simple two-step procedure performed in a microtiter plate. In the first step samples are incubated with the HDAC assay substrate, allowing deacetylation of the fluorometric substrate. Next, Activator Solution releases a fluorophore from the deacetylated substrate or standard.
