Want to know if Cas9 is active in your cells? Cellecta’s CRISPRtest™ method allows you to assess the level of Cas9 activity in your cell line using two pre-mixed lentiviral-packaged vectors, the first containing a green fluorescent protein (GFP) marker and gRNA sequence targeting an essential gene (PCNA), and the second containing a red fluorescent protein (RFP) marker and a non-targeting gRNA. Transduction of CRI…
Cellecta’s Ready-to-Use Lentiviral Packaging Plasmid Mix allows the production of pseudoviral particles when mixed with second- or third-generation lentiviral vectors. The Packaging Plasmid Mix is a mixture of the human immunodeficiency virus (HIV) lentiviral packaging plasmid psPAX2 and the pMD2.G plasmid containing VSV-G. These plasmids provide all the necessary structural, regulatory, and replication proteins required to…
LentiFuge™ viral concentration provides a simple, rapid, and effective method to increase the amount of lentiviral particles collected from cell culture supernatants using standard high-speed centrifugation. It works by aggregating viral particles so that upon high speed centrifugation, so the virus can be precipitated and isolated without use of an ultracentrifuge. LentiFuge is a sterile, cell culture grade reagent that is…
Optimized for brewing yeast, wine yeast and other small cells with one channel of fluorescence.
Cell viability and vitality kit to measure metabolic activity and dead/non-viable nucleated cells with Hoechst to determine total nucleated cell count.
Membrane-exclusion, DNA binding dye to detect dead and/or necrotic cells
Real-time measurement of apoptosis in 2D culture and 3D tumor spheroids
Real-time measurement of apoptosis in 2D culture and 3D tumor spheroids, with Hoechst to determine total number of cells
Fluorescein active Caspase-3 staining kit for apoptosis
Fluorescein active Caspase-3 staining kit for apoptosis
Fluorescein active Caspase-8 staining kit for apoptosis
