Chromatin Immunoprecipitation (ChIP) is an important technique allowing the researcher to analyze in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powe…
The EZ-Zyme™ Chromatin Prep kit allows ChIP analysis at nucleosome resolution by performing complete or partial digestions with a proprietary enzymatic cocktail to obtain chromatin fragments of on average one to a few nucleosomes in length. Compared to sonication, this procedure not only allows mapping the protein-DNA association at a higher resolution, but also renders the subsequent immunoprecipitation more efficient due to…
Sterivex filter units work with syringes, peristaltic pumps or pressure vessels. They eliminate the risk of contamination associated with decanting or pipetting.Sterivex units are designed to dispense into virtually any storage container. The devices are available with different outlet connectionsSterivex filter units can process volumes up to 1,000 mL or 2,000 mL, depending on the type of membrane used in the device. A choice…
The Steriflip filter unit is designed to work with standard 50 mL centrifuge tubes, which eliminates sample transfer. To filter, just attach the unit to a 50 mL centrifuge tube containing your sample, flip it over, and apply the vacuum. When finished, the filtrate collects in the attached 50 mL centrifuge tube.Steriflip Nylon Net filters are now available in 40, 60 and 100 µm pore sizes, making them ideal for stem cell, cardio…
Sterivac vacuum-driven filter units are ideal for bottle-top filtering directly into receiver flasks at flow rates as fast as 1.5 L/min. The unique "start & stop" filtration action makes setting up, filtering and transferring the unit to another receiver fast and easy. Push a button to stop filtering, move the device to an empty bottle and restart
The Stericap â„¢ PLUS Universal Bottle-Top Filter Device provides fast, large-volume filtration into any sized bottle.
• Concentrating and desaltingchromatography column eluates• Concentrating monoclonal antibodies• Concentrating proteins or viruses from culture supernatants• Clarifying tissue homogenates and cell lysates• Buffer exchange or diafiltration
• High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs• High retentate recovery of >90%• Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)• Heat-sealed membrane minimizes downstream extractables• 100% integrity tested for reliable performance• Convenient sample monitoring with translucent housing and volume gradations• Direct pipet…
• High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs• High retentate recovery of >90%• Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes)• Heat-sealed membrane minimizes downstream extractables• 100% integrity tested for reliable performance• Convenient sample monitoring with translucent housing and volume gradations• Direct pipette s…
Easy, pre-sterilized, centrifugal sample clarification units for either 0.5 mL (MC) or 2 mL (CL) maximum volumes.High recovery Durapore (PVDF) membraneFast filtration and highly reproducible performanceUse in fixed-angle rotors for 1.5 mL tubes (MC) or 15 mL tubes (CL)
Montage Antibody Purification Kits are designed for fast and easy antibody purification from serum, ascites or cell culture supernatants. The kits include spin columns pre-packed with high capacity PROSEP-A or G media. There are no pumps to setup. No columns to pack. All you need is a centrifuge to purify 10–20 mg in less than 60 minutes.The protocol is easy to follow and, unlike traditional gravity flow methods, the use of ce…
With their vertical membrane design, Amicon Ultra 0.5 mL filters deliver great performance and lower spin timesConcentrates 500 µL down to 15 µLConcentration factor 25 x to 30 xHigh sample recovery (greater than 90% of dilute starting solution)Fast processing time - 10 to 30 minutesConsistent recoveries thanks to the reverse spin capabilityReverse spin capability provides consistent recoveries
With their vertical membrane design, Amicon Ultra 2 mL filters deliver the highest recovery with shortest spin times• Concentrates 2 mL down to 15-70 µl• 98% sample recovery• Fast processing time - 10 to 30 minute spin times• Engineered dead stop• Reverse spin capability provides consistent recoveries
Concentrates 2 mL down to 15-70 µL 98% sample recovery Fast processing time - 10 to 30 minute spin times Engineered dead stop Reverse spin capability provides consistent recoveries
Automation compatible filter units from EMD Millipore are specially designed to provide efficient filtration for dissolution and other automated sample preparation applications.Choice of Filter Media: Automation compatible filter units are available in a variety of membrane types and pore sizes, including units with prefilters for particulate-laden samples.Automation Compatible Packaging: The 200 pack comes preassembled in 8 t…
Millex filter units with hydrophobic Fluoropore membrane are ideal for sterilizing gases, venting sterile containers, and sterilizing or clarifying organic solutions. Specialized filter units protect hemodialysis transducers from blood and moisture. 50 mm Millex filter units are also used for vacuum line protection. All units are bi-directional
This 13 mm syringe filter with a Hydrophilic Polyethersulfone membrane, for non-sterile filtration, has a 0.45 micron pore size used in preparation of aqueous & mild organic solutions prior to chromatography or other instrument analyses.
4 mm syringe filter with a Hydrophilic PVDF membrane, for non-sterile filtration, has a 0.22 µm pore size used in fine particle removal from aqueous & mild organic solutions.
Millex-GP Filter for Samplicity G2, 0.22 µm, PES 33 mm, non-sterile
The CpGenome™ DNA Modification Kit is used to create methylation-specific sequence alterations in any DNA sample. Using chemical treatment, the kit converts all unmethylated cytosine residues to uracil. Methylated cytosine residues are unaltered in this process. Therefore, the chemically-treated DNA sequence will differ only if it was originally unmethylated in vivo. After sequence conversion, methylation specific PCR is emplo…
The CpGenomeâ„¢ Fast DNA Modification Kit contains the reagents for the initial bisulfite modification of the DNA required for both methodologies.
Bruker Spectrometry Solutions ensure nondestructive and environmentally clean and safe multi-element analyses for Process and Quality Control or in Research and Development.
ChIP-Seq facilitates genome-wide profiling of the interactions of transcription factors, modified histones, and other chromatin associated proteins. By combining chromatin immunoprecipitation with next generation sequencing, laboratories can generate high resolution data on protein-DNA interactions across the genome. For laboratories interested in gaining a deeper understanding of epigenetic mechanisms of gene regulation, ChIP…
