The Thermo Scientific ABgene 6.0ml Storage Plate is suitable for storage applications where large sample volumes are required. Features • 6.0ml working volume when sealed with adhesive or heat seals • 48 square wells • Pyramidal well bottom for ease of sample retrieval • Support and stacking ribs
Thermo Scientific Gene Frame products feature a dual strength adhesive system which facilitates the easy removal of the frames after use. The adhesives on either side of the frame are of differing strengths; the side which adheres to the glass has an easy release adhesive whereas the reverse, which sticks to the coverslip, forms a stronger bond. This means that when the coverslip is removed, due to the disparity in the strengt…
Thermo Scientific Superladders are derived from cloned and purified DNA fragment repeats. A range of sizes are available. Superladder-Low 20bp LadderSize range: 20-1000bpNumber of bands: 50 Superladder-Low 100bp Ladder Size range: 100-1000bp Number of bands: 10Superladder-Mid 100bp Ladder Size range: 100-3000bp Number of bands: 30Superladder-Mid 200bp Ladder Size range: 200-6000bp Number of bands: 30
Thermo Scientific MMuLV (Moloney Murine Leukemia Virus) Reverse Transcriptase synthesizes a complementary DNA strand from single-stranded RNA. It is isolated from a clone over-expressing the gene from Moloney Murine Leukemia Virus. Features Good dynamic range Low RNase H activity ApplicationscDNA SynthesisRT-PCR
Thermo Scientific has a wide range of DNA digest markers for low and high molecular weight products. Markers are supplied with loading dye allowing the same loading dye to be used for the sample and the marker. Markers available:PhiX174 RF DNA Hae III MarkerPhiX174 RF DNA Hinc II MarkerPhiX174 RF DNA Hinf I MarkerpBR322 hae III MarkerpBR322 Msp I MarkerLambda Hind III MarkerLambda EcoRI/ Hind III Marker
The Thermo Scientific Dye Terminator Removal Kit is designed to perform the essential removal of unbound fluorescently-labelled dideoxy-ribonucleotides (ddNTPs) and excess salt from sequencing reactions prior to sequence analysis. The kit includes a 96-well plate containing pre-hydrated gel separation matrix consisting of spheres with uniformly sized pores. Sequencing reactions are passed through the separation matrix, which r…
The Thermo Scientific Separation Plate can be used for gel filtration applications (eg. dye terminator removal) when a suitable matrix is added. Features • 96 wells containing polyethylene frits, pore size of 30–45µm • 0.6ml well volume • Ready-to-use microplate format • Suitable for centrifugation
Thermo Scientific RNAse Inhibitor specifically inhibits some of the most common types of RNases making it the perfect solution to protect your RNA. Thermo Scientific RNase Inhibitor is isolated by affinity chromatography from porcine livers. This enzyme acts on type A RNases (RNase A, B and C) and functions in any enzymatic reaction buffer with pH 5-8 (optimal activity is observed at pH 7-8). Porcine RNase Inhibitor does NOT i…
Thermo Scientific Exonuclease I digests single-stranded DNA 3' to 5' (requires Mg2+ and a free 3'-OH). The nuclease is inactivated by heating at 80°C for 15 minutes.
Thermo Scientific rDNase I is produced through a process that contains no animal by-products. The enzyme is provided free from any detectable RNase activity or animal derivatives. rDNase I has a molecular weight of approximately 30kDa. As an endodeoxyribonuclease, DNase I hydrolyses phosphodiester linkages yielding di-, tri-, and oligonucleotides with a 5' phosphate and a free 3' hydroxyl. DNase I has been shown to act on sing…
Thermo Scientific Shrimp Nuclease is an endonuclease that cleaves phosphodiester linkages in DNA to yield di- and oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. The enzyme has a high preference for double-stranded DNA, and can be used to selectively degrade dsDNA, leaving ssDNA and RNA intact. The enzyme is inactivated after 15 minutes at 65 °C.
Thermo Scientific RNase A (ribonuclease A) is supplied as a chromatographically pure freeze-dried powder, salt-free for easy reconstitution in most buffers.
Thermo Scientific Ribonuclease H (RNase H) is an endonuclease that degrades the RNA portion of DNA-RNA hybrids. It hydrolyses phosphodiester bonds of RNA in RNA:DNA heteroduplexes. It produces products with 3’-OH and 5’-phosphate. RNase H will not degrade single or double-stranded DNA or RNA.
Thermo Scientific Shrimp Alkaline Phosphatase (SAP) catalyzes the dephosphorylation of 5´ phosphates from DNA and RNA and is completely and irreversibly inactivated by heating at 65°C for 15 minutes. SAP is active on 5´ overhangs, 5´ recessed and blunt ends.
Thermo Scientific IPTG (isopropyl-ß-D-thiogalactopyranoside) is a chemical analogue of galactose, which cannot be cleaved by ß-galactosidase. It acts as an inducer of the lac operon.
The Power SYBR® Green RNA-to-CT™ 1-Step Kit provides high sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction kit. A novel formulation helps reduce false-positive results caused by primer-dimers that are common in SYBR® Green-based assays. This significantly improves data accuracy and enables the reliable detection of low expressing targets or targets present at low amounts. Minimized hands-on time wit…
Power SYBR® Green RNA-to-CT 2-Step Kit is a flexible kit that enables a two-step protocol for performing reverse transcription and cDNA quantitation by real-time PCR. The kit includes the High Capacity RNA-to-cDNA and the PowerSYBR® Green PCR Master Mix. The High Capacity RNA-to-cDNA Kit is used to first reverse transcribe RNA into cDNA and the resulting cDNA is then quantitated using the PowerSYBR® Green PCR Master Mix for…
The SYBR Green RT-PCR Kit provides all the necessary components to perform both one- and two-step RT-PCR, which makes it ideal for evaluation between the two RT-PCR protocols.
High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with the TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix and others. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template quantities. The new High Capacity RNA-to-cDNA formulation reduces reverse transcription time by…
The High Capacity RNA-to-cDNA Master Mix optimizes cDNA synthesis for high-throughput, real-time gene expression analysis. • Single tube, complete first-strand cDNA synthesis master mix reduces experimental variability and streamlines workflow • 5X concentration provides robust results even with dilute RNA samples and rare or abundant transcripts • Liquid format at –10 and –20°C eliminates freeze/thaw cycles and maximizes chem…
Ambion Anti-miR™ miRNA Inhibitors are chemically-modified, single-stranded nucleic acids designed to specifically bind to and inhibit endogenous miRNA molecules. These ready-to-use inhibitors can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs, and enable detailed study of miRNA biological effects.
Whether your profiling experiment requires ultimate sensitivity, broad coverage or both, Megaplex™ Primer Pools provide the flexibility to reach your research goals. Megaplex Primer Pools deliver comprehensive coverage of Sanger miRBase v10, and when used with TaqMan® MicroRNA Arrays, offer an ideal miRNA profiling solution. Optional Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed miRNAs en…
Ambion Pre-miR™ miRNA Precursor Molecules are small, chemically-modified double-stranded RNA molecules designed to mimic endogenous mature miRNA molecules. These ready-to-use miRNA mimics, which are similar to but not identical to siRNAs, can be introduced into cells using transfection or electroporation parameters identical to those used for siRNAs and enable detailed study of miRNA biological effects via gain-of-function e…
Silencer Pre-Designed siRNAs are Ambion-designed siRNAs available for all human, mouse, and rat gene targets in the RefSeq database. These siRNAs are designed for maximum potency and specificity using a highly effective and extensively tested algorithm. Each siRNA is synthesized to the highest quality standards and is provided with full sequence information. Silencer Validated siRNAs are Ambion-designed siRNAs that have been…
Ambion Silencer Select Pre-Designed and Validated siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNA with unrivaled efficacy, potency, and specificity. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data.
