Promega Corp. Forensics Products & Reviews

Extracts RNA from Tissues, Cultured Cells and White Blood Cells

Full-Length cDNA Synthesis for the Analysis of Rare or Long Messages

Single-Use Ligands for High-Resolution lmaging in Live Cells

Extract high-quality nucleic acids with less hands-on time.

High-Throughput Extraction of DNA from FFPE Samples Robust, precipitation-free protocol, no chance of lost pellets High yields of pure DNA from FFPE samples without using xylene or other hazardous chemicals Ideal for use in downstream applications including qPCR and next-generation sequencing (NGS)

The SV 96 Total RNA Isolation System provides a high-throughput technique to prepare intact RNA from tissue and cultured cells. Total RNA can be purified from 96 samples in less than an hour without centrifugation. The system incorporates a DNase treatment step that is designed to substantially reduce genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without phenol:…

Column-based method for rapid concentration and cleanup of DNA.

Column-based method for rapid concentration and cleanup of RNA.

Optimized PCR master mix: Just add template, primers and water

Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading ac…

New Site-Specific Endoprotease that Targets Proline and Alanine

The PureYield™ Plasmid Midiprep System is designed to isolate transfection-quality plasmid DNA.   The system provides a rapid method for purification of 100–200μg of plasmid DNA from 50ml bacteria culture. Plasmid DNA can be purified in as little as 30 minutes with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows…

GoTaq®2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) method. The first step consists of cDNA synthesis using the GoScript™ Reverse Transcription System. Optimized reagents and ultra-active, GoScript™ Reverse Transcriptase result in high-efficiency, full-length cDNA synthesis from rare and abundant targets with up to 5 µg of RNA inp…

GoTaq® qPCR Master Mix is a new reagent system for use in real-time quantitative PCR (qPCR). The system contains BRYT™ Green, a new proprietary fluorescent DNA binding dye that exhibits greater fluorescence enhancement upon binding to double-stranded DNA than SYBR® Green I. Combined with the GoTaq® Hot Start Polymerase, optimized buffer and proprietary dye, GoTaq® qPCR Master Mix provides robust real-time PCR with increased re…

GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The system is supplied with a tube of 25mM MgCl2, allowing optimization of the magnesium concentration in your reactions. It is also supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer. The buffers contain a compound that increases sample density, so tha…

The GoScript™ Reverse Transcription System includes a reverse transcriptase and a specialized set of reagents for efficient synthesis of first-strand cDNA optimized for quantitative PCR amplification. GoScript™ Reverse Transcriptase uses M-MLV Reverse Transcriptase and state-of-the-art buffer technology to deliver robust, reliable cDNA synthesis of a full range of rare and abundant transcripts, even in the presence of inhibit…