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BL21 Competent E. coli

New England Biolabs Inc.

BL21 Competent E. coli is a widely used non-T7 expression E. coli strain and is suitable for transformation and protein expression. This strain does not express the T7 RNA Polymerase. Ideal for Plac, Ptac, Ptrc ParaBAD expression vectors Protease deficient No dry ice surcharge on competent cell shipments Outgrowth medium included Free of animal products

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Lambda Exonuclease

New England Biolabs Inc.

DNA specific exonuclease Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 5' to 3' direction Highly processive degradation of double-stranded DNA from the 5' end Preferred substrate is 5'-phosphorylated double-stranded DNA although non-phosphorylated substrates are degraded at a greatly reduced rate Lambda Exonuclease is ideal for: Conversion of linear double-stranded DN…

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1 kb DNA Ladder

New England Biolabs Inc.

A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5-10.0 kilobases (kb). The 3.0 kb fragment has increased intensity to serve as a reference band. The approximate mass of DNA in each of the bands is provided (assuming a 0.5 μg load) f…

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