High-quality ligase. KAPA T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA. This enzyme efficiently joins blunt and cohesive end termini, as well as repairs single-stranded nicks in duplex DNA, RNA, and DNA/RNA hybrids. Applications: Routine subcloning Recircularization of linear DNA Library construction Linker ligation
Fast and efficient ligation. The KAPA Rapid Ligation System is a combination of KAPA Rapid T4 DNA Ligase and an optimized reaction buffer designated to ligate blunt or cohesive end DNA fragments in 5 minutes at room temperature. KAPA Rapid Ligase contains T4 DNA Ligase, which catalyzes the formation of phosphodiester bond between 5′-phosphate and 3′-hydroxyl termini in duplex DNA or RNA. Features: 5-minute ligati…
The KAPA Stranded RNA-Seq Kit with RiboErase offers a high-quality, comprehensive solution for transcriptome sequencing. By utilizing a targeted enzymatic method for depletion, our workflow enables superior reduction of ribosomal RNA (rRNA) and a more complete representation of the transcriptome, including precursor mRNAs and non-coding RNA (ncRNA). Kits also contain KAPA HiFi for high-efficiency and low-bias library amplifica…
