Imaging Technology for Biomedical Microscopy 3i designs and manufactures optical microscope systems, components, and software for cutting edge biomedical research. Their experienced team of scientists and engineers continue our 15-year tradition of creating advanced research instruments. 3i provides an array of microscopy platforms to suit needs from the individual researcher to core facilities. Their modular systems can be expanded with an array of technologies to address both established and emergent scientific methods. Their over-arching goal is to continue to create the best imaging technologies by scientists for scientists.
The Marianas™ research microscopy imaging system combines state-of-the-art optics, custom electronics, detectors, and computers for both live and fixed cell applications. 3i’s SlideBook™ software takes full advantage of the system's automation by seamlessly integrating components, including x-,y- and z-stage motion, filter control and light shuttering. Methods: • 7D imaging • FRET • TIRF • FLIM • Ratiometric imaging • Photoman…
SlideBook™ software carries biomedical imaging through the entire experimental process. By addressing everything from device control to image processing to automated data analysis, SlideBook™ allows users to focus on scientific investigation and novel methods rather than on core instrument functionality. SlideBook™ 5 comes standard with a wide variety of acquisition drivers to control over 100 devices from dozens of companies.…
3i combines the Yokogawa CSU-X1 with proprietary high speed blanking electronics to achieve imaging speeds up to 2000 fps. The microlens double-Nipkow disk design of the spinning disk is ideal for high-speed 3D live cell imaging. Powerful and stable solid state lasers from the 3i LaserStack™ are coupled through the microlens pinholes of the spinning disk providing strong excitation light and high signal-to-noise images. SlideB…
The LaserStack™ laser launch features solid state diode lasers and AOTF beam selection in a modular, vertically integrated design to allow configuration of a laser launch for current applications and later expand it as new applications require. LaserStack™ can accommodate the following wavelengths: 405 nm, 445 nm, 473 nm, 488 nm, 515 nm, 532 nm, 561 nm, 640 nm, 660 nm. LaserStack™ outputs in a single mode fiber for use in spin…
Vector™ is a diffraction limited high-speed x,y scanner that enables a number of investigative methods from quantitative photobleaching analysis to direct specimen manipulation techniques such as photoablation. Vector™ is also designed for high-speed scanning imaging techniques, such as multiphoton imaging or laser scanning confocal microscopy.
Vivo™ is a research microscopy imaging system ideal for intravital imaging and electrophysiology. Designed for the visualization of metabolic processes in live cells, Vivo™ facilitates quantitative measurement of dynamic processes in a light-starved environment. 3i’s SlideBook™ software takes full advantage of the system's automation by seamlessly integrating components, including x-,y- and z-stage motion, filter control and l…
Vivo 2-Photon™ is a multiphoton microscopy system for high-speed deep tissue imaging. Key to the Vivo 2-Photon™ is its ability to operate additionally as a widefield microscopy imaging system. Modulation of laser power with focus depth enables the collection of multiple volumes per second with minimal photobleaching. 3i’s SlideBook™ software takes full advantage of the system's automation by seamlessly integrating components,…
The Everest™ research microscopy imaging system combines state-of-the-art optics, custom electronics, detectors, and computers for fixed cell applications. 3i’s SlideBook™ software takes full advantage of the system's automation by seamlessly integrating components, including x-,y- and z-stage motion, filter control and light shuttering. Methods • 7D imaging • FRET • TIRF • FLIM • Photomanipulation o FRAP o FLIP o Selective il…
The mSwitcher™ is a galvo-based port switcher that can support up to 3 imaging devices and allows millisecond switching between confocal, ablation, and TIRF modalities.
The mSAC™ is a high-speed spherical aberration correction device. Similar to an objective correction caller, the mSAC™ corrects for spherical aberration in thick samples and updates dynamically as focus depth is increased. The mSAC™ can be used with all objectives on a microscope.
The Ablate!™ is an ablation laser system with attenuatable power and rapid scanning (1000 pulses per second) capable of inflicting localized damage to intracellular structures, ideal in precision cutting for targeted wound control. Ablate!™ enables photo-ablation, photo-cutting, and photo-wounding utilizing a 532 nm laser pulsed at up to 70 µJ at up to 1 kHz.
