Intelligent Imaging Innovations (3i) Products & Reviews

The Marianas™ research microscopy imaging system combines state-of-the-art optics, custom electronics, detectors, and computers for both live and fixed cell applications. 3i’s SlideBook™ software takes full advantage of the system's automation by seamlessly integrating components, including x-,y- and z-stage motion, filter control and light shuttering. Methods: • 7D imaging • FRET • TIRF • FLIM • Ratiometric imaging • Photoman…

SlideBook™ software carries biomedical imaging through the entire experimental process. By addressing everything from device control to image processing to automated data analysis, SlideBook™ allows users to focus on scientific investigation and novel methods rather than on core instrument functionality. SlideBook™ 5 comes standard with a wide variety of acquisition drivers to control over 100 devices from dozens of companies.…

3i combines the Yokogawa CSU-X1 with proprietary high speed blanking electronics to achieve imaging speeds up to 2000 fps. The microlens double-Nipkow disk design of the spinning disk is ideal for high-speed 3D live cell imaging. Powerful and stable solid state lasers from the 3i LaserStack™ are coupled through the microlens pinholes of the spinning disk providing strong excitation light and high signal-to-noise images. SlideB…

The LaserStack™ laser launch features solid state diode lasers and AOTF beam selection in a modular, vertically integrated design to allow configuration of a laser launch for current applications and later expand it as new applications require. LaserStack™ can accommodate the following wavelengths: 405 nm, 445 nm, 473 nm, 488 nm, 515 nm, 532 nm, 561 nm, 640 nm, 660 nm. LaserStack™ outputs in a single mode fiber for use in spin…

The Everest™ research microscopy imaging system combines state-of-the-art optics, custom electronics, detectors, and computers for fixed cell applications. 3i’s SlideBook™ software takes full advantage of the system's automation by seamlessly integrating components, including x-,y- and z-stage motion, filter control and light shuttering. Methods • 7D imaging • FRET • TIRF • FLIM • Photomanipulation o FRAP o FLIP o Selective il…

The mSwitcher™ is a galvo-based port switcher that can support up to 3 imaging devices and allows millisecond switching between confocal, ablation, and TIRF modalities.

The mSAC™ is a high-speed spherical aberration correction device. Similar to an objective correction caller, the mSAC™ corrects for spherical aberration in thick samples and updates dynamically as focus depth is increased. The mSAC™ can be used with all objectives on a microscope.

The Ablate!™ is an ablation laser system with attenuatable power and rapid scanning (1000 pulses per second) capable of inflicting localized damage to intracellular structures, ideal in precision cutting for targeted wound control. Ablate!™ enables photo-ablation, photo-cutting, and photo-wounding utilizing a 532 nm laser pulsed at up to 70 µJ at up to 1 kHz.