Agilent Technologies Drug discovery > Basic Research Products & Reviews

The Agilent CE/MS System comprises the Agilent 7100 CE System directly interfaced with any of the Agilent LC/MS systems. A range of ion sources, including standard ESI, can be installed, and additional detectors—such as UV-DAD, LIF, and CCD—can be operated parallel to MS. Agilent MassHunter software can be used to control the entire system from one workstation.

The Agilent DNA Fish ID solution uses advanced software to achieve more accurate results – quickly and easily. Key to the process is the Agilent 2100 Bioanalyzer and RFLP Decoder Software performs RFLP pattern matching, which eliminates the need for manual calling

The StrataPrep plasmid preparation kits provide a rapid method for purifying plasmid DNA from bacterial cultures.

The Affinity protein expression and purification system provides a simple, gentle and effective method for high-level protein expression and purification. The complete system includes pCAL expression vectors containing a calmodulin-binding peptide tag and calmodulin affinity resin.

Three different strains that provide varying levels of expression control with T7 promoter-driven vectors, such as the pCAL and pET vectors.BL21 derivatives for tight expression control Express toxic proteins Use BL21, BL21-Gold or BL21-CodonPlus cells High-level protein expression Use for established expression constructs Economical alternative

BL21-CodonPlus strains are engineered to contain extra copies of genes that encode the tRNAs that most frequently limit translation of heterologous proteins in E. coli. They are ideal for difficult protein expression, especially when codon bias is a problem.

The pET E. coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene.

Allows in vivo excision of cloned fragments in the phagemid vector Screening with both antibodies and nucleic acid probes Each premade library comes with necessary strains and helper phage  

This collection of competent cells provides customers with classic  E. coli  strains that have been engineered to become competent for use in cloning experiments.

Identify fish species quickly, easily, and accurately. Whether your samples are fresh, frozen, dried, salted, or minced, Agilent’s DNA Fish Species Identification solution provides reliable, robust answers within hours. And, the method is far more accurate than looking at a fish and guessing.

The pBK-CMV vector allows you to express proteins in both E. coli and mammalian model systems and saves you valuable time by not having to subclone into an additional vector.  In mammalian cells, the expression is driven by the CMV promoter and is therefore constitutively expressed.  In E. coli, the lac promoter drives high-level expression in the presence of the inducer IPTG, and allows minimal expression in the absence of i…

The Complete Control Inducible Mammalian Expression System is designed to provide the tightest control of expression available in mammalian cells. The system is based on a synthetic ecdysone-inducible receptor and a synthetic receptor recognition element that modulates expression of your gene of interest.  

The LacSwitch II inducible mammalian expression system utilizes an improved vector system in which several elements of the lac operon have been modified for use in eukaryotic cells for the control of gene expression.

Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study. The pCMV-Tag vectors have a single copy of the available epitope tags, while the pCMV-3Tag vectors have three copies of the epitope tag (in a variety of configurations) to prove a stronger signal in immmunological assays.

Method completed in less than one day versus 2 days with restriction enzymes

The pSG5 Vector is a 4.1 Kb eukaryotic expression vector constructed by combining pKCR2 and the Stratagene pBS vector. The high copy number of this plasmid allows for large quantities of double-stranded DNA to be obtained. Expression in vivo is achieved via transient expression in a variety of cell lines (highest level of expression is obtained following transfection of a cell line expressing the T antigen).

StrataClean resin eliminates the need to perform phenol:chloroform extractions, which are highly toxic and combustible. The resin is a non-toxic, non-combustible slurry of hydroxylated silica particles that is used to clean up DNA or concentrate dilute protein mixtures.

The StrataClone Mammalian Expression Vectors were designed for high-level expression of epitope-tagged proteins in mammalian cells. Use of StrataClone technology for the cloning steps allows rapid cloning of blunt-ended PCR products, produced using high-fidelity PCR enzymes, into the epitope tagging/expression vectors.

Restriction Enzymes Dpn I is an ideal enzyme for cloning and Southern blot analysis High purity enzyme preparation Includes 10X universal reaction buffer Glogos II Autorad Markers Luminescent peel-off stickers used for accurately marking and aligning autoradiographs. Easily affixed to plastic wrap or paper, these convenient markers emit low-level luminescence rather than radioactivity, making the Glogos II m…

Our Mycoplasma Plus PCR Primer Set detects infection by all forms of Mycoplasma with a robust single-band PCR product (triplet for Acholeplasma). This kit also includes controls to verify PCR amplification and Mycoplasma genomic DNA. The primer design permits higher annealing temperatures, reducing spurious bands from PCR contaminants.

The MycoSensor PCR & QPCR Assay Kits detect Mycoplasma infection of cell cultures by real-time quantitative PCR (qPCR) or PCR in less than two hours.

Our SideStep lysis and Stabilization buffer ensures accurate gene expression studies by eliminating sample loss and degradation. Our proprietary buffer lyses cells and immediately stabilizes the released nucleic acid for months. Following the 10-minute room temperature lysis, you can go directly from cells to QPCR amplification without stopping to purify. 

pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.

The Mx3005P system is among the most reliable and trusted QPCR instruments available, with a long record of citation in peer-review journals. The Agilent Mx3005P and Mx3000P QPCR Systems are the most flexible—and reliable—instruments available for gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis, and chromatin immuno-precipitation studies.Highly reproducible re…

Mx Pro QPCR software with FDA guideline 21 CFR Part 11 compatible features. Four key elements have been added to create the ET software version; secure application login, database file management, electronic audit trail, and report generation.