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Trypsin/Lys-C Mix, Mass Spec Grade

Promega Corp.V5071, V5073, V5072

Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution. Using the conventional trypsin digestion protocol (i.e., overnight incubation at nondenaturing conditions), Trypsin/Lys-C Mix improves protein digestion by eliminating the majority of missed cleavages,…

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We regularly buy in bulk!

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Application Area:

Routine mass spectrometry

Very easy to use - we find this trypsin/LysC to be incredibly reliable.

Review Date: 25 Oct 2019 | Promega Corp.

Always works perfectly. Never fails and is never contaminated.

Average Rating 5.0

Application Area:

Peptide mapping

In our lab we only use Promega Trypson/Lys-C. We've been using these reagents for over 9 years without ever having an issue attributable to the reagent. Not the same with all other protease suppliers.

Review Date: 27 Jun 2019 | Promega Corp.

Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution.

Using the conventional trypsin digestion protocol (i.e., overnight incubation at nondenaturing conditions), Trypsin/Lys-C Mix improves protein digestion by eliminating the majority of missed cleavages, which occur at prominent quantities in trypsin digests. Trypsin/Lys-C Mix enhances digestion and compensates for the trypsin proteolytic inefficiency at lysine sites.

Trypsin/Lys-C Mix, Mass Spec Grade Features & Benefits:

Simple to Use - Use standard overnight digestion with nondenaturing conditions.
Enhanced Proteolysis - Increase peptide recovery, resulting in enhanced protein quantitation and improved reproducibility and eliminating the majority of missed cleavages (Figures 1 and 2).
Tolerant to Trypsin-Inhibiting Contaminants - Generate mass spectrometry data from poor-quality sample material.

Application NoteSeparations

Reference Yeast and Human Whole Cell Protein Extracts for Mass Spectrometry Instrument Performance Monitoring and Method Development

This application note, two protein extraction formats are described: pre-digested extracts, for monitoring instrument performance, and intact protein extracts, for optimization of sample preparation. To be compatible with mass spectrometry applications, extracts need to meet certain requirements, like freedom from detergents, high protein integrity and high reproducibility. Here, whole cell protein extracts from yeast and human cells that meet those criteria are described.

8 Jul 2015

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Trypsin/Lys-C Mix, Mass Spec Grade - Promega Corp. - Life Sciences

Trypsin/Lys-C Mix, Mass Spec Grade

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