TransOmics™ Solution

Fatty acids, both free and as part of complex lipids, play a number of key roles in metabolism – as major metabolic fuel (storage and transport of energy), as essential components of all membranes, and as gene regulators. In LC/MS methods, although no sample derivatization is required, the runs typically involve labor-intensive and time-consuming sample preparation, and utilize toxic organic solvents, which are expensive to purchase and dispose. As described in this application note, the analysis of fatty acids in the free form instead of FAME derivatives results in easier and faster sample preparation. The organic phase extract containing all the FFA can be injected directly into the system, which results in significant savings in sample preparation and analysis time, solvent costs, and solvent waste disposal.

This application note presents a method combining HILIC-UPLC® liquid phase separation with gas phase ion mobility mass spectrometry for the multi-dimensional characterization of lipids in complex mixtures. The method enhances profiling of lipids in biological samples.

This application note describes a multi-omics solution for the large-scale analysis of MS data from metabolomics and proteomics data sets. The Waters Omics Research Platform Solutions with TransOmics Informatics, featuring the Xevo G2-S QTof System, was utilized in a study comprised of both technical and biological replicates.

Use of the Waters Omics Research Platform, which combines UPLC® with ion mobility LC/MSE and TransOmics™ Informatics, allows for the rapid characterization of molecular phenotypes of plants that are exposed to different environmental stimuli.