SURVEYOR® Mutation Detection Kit for Standard Gel Electrophoresis

SURVEYOR Mutation Detection Kits provide a simple, accurate and cost-effective means to scan DNA fragments for mutations with unmatched sensitivity and specificity.

The SURVEYOR Mutation Detection Kit for Standard Gel Electrophoresis has been designed to cleave unlabeled DNA fragments at mismatched sites for subsequent analysis by agarose gel electrophoresis or polyacrylamide gel electrophoresis (PAGE). DNA 200 to 4,000 bp long can be analyzed using manual agarose gel electrophoresis while smaller fragments (<1,000 bp) can be analyzed using manual polyacrylamide gel electrophoresis (PAGE).


Benefits:

• Determines mutation loci
• Saves time and money
  – Streamlines DNA sequencing
  – Decreases sequencing backlog and analysis time
  – Validates sequencing results and analysis
  – Decreases chromatogram analysis by 90%
• No post-PCR or post-reaction clean-up
• High throughput sensitivity and specificity

Applications:

• Somatic, germ-line and mitochondrial mutations
• Insertions, deletions, substitutions and SNPs
• Low-copy mutations and pooled DNA samples