SialEXO™
SialEXO is a sialidase mix for complete removal of sialic acids from native glycoproteins. SialEXO is supplied as a lyophilized powder with no preservatives added. It is available in 2000 unit vials for release of sialic acids on 2 mg glycoprotein.
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SialEXO is a combination of two sialidases for efficient desialylation of O- and N-glycosylated proteins. The mix is composed of sialidases for high efficiency in hydrolyzing α2-3, α2-6 or α2-8 bonds. SialEXO hydrolyzes glycoproteins under native conditions and displays activity in a pH range from 6.5 to 9. Enzymes in SialEXO are derived from Akkermansia muciniphila and expressed in E. coli. The enzymes in the mix contain a His-tag and the molecular weights of the components are 43 kDa and 66 kDa, respectively.
Unit Definition
One unit of SialEXO hydrolyzes sialic acids from ≥ 90% of 1 µg glycoprotein (fetuin) when incubated in 20 mM Tris pH 6.8 at 37 °C for 2 h.
Content and Storage
SialEXO is supplied lyophilized in TBS pH 7.6, with no preservatives added.
SialEXO is shipped cold and should be stored at –20 °C upon arrival.
After reconstitution SialEXO is stable for 1 month at +4-8 °C.
