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SeQuant® ZIC®-HILIC HPLC Columns

Name: SeQuant® ZIC®-HILIC HPLC Columns
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ZIC®-HILIC HPLC columns for the separation of all classes of polar and hydrophilic compounds. What is HILIC? HILIC or Hydrophilic Interaction Liquid Chromatography is a straightforward chromatographic technique for separation of many types of polar and hydrophilic compounds. To put it simple one can say that HILIC is a normal-phase (NPLC) type of separation but uses reversed-phase (RPLC) type eluents. In HILIC one has: A c…

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Description

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ZIC®-HILIC HPLC columns for the separation of all classes of polar and hydrophilic compounds.

What is HILIC?

HILIC or Hydrophilic Interaction Liquid Chromatography is a straightforward chromatographic technique for separation of many types of polar and hydrophilic compounds. To put it simple one can say that HILIC is a normal-phase (NPLC) type of separation but uses reversed-phase (RPLC) type eluents.

In HILIC one has:

A column with a hydrophilic stationary phase
An eluent with water, buffer and a high concentration of water-miscible organic solvent

Analytes such as amino acids, peptides, carbohydrates, plant extracts and various other polar compounds that usually have little or no retention and may be affected by wettability problems in RPLC, generally have strong retention on the ZIC®-HILIC column.

The zwitterionic ZIC®-HILIC stationary phase is attached to porous silica and is available in columns from capillary to preparative dimensions. The separation is achieved by a hydrophilic partitioning mechanism superimposed on weak electrostatic interactions.

Improved separation of hydrophilic and polar compounds
Orthogonal selectivity to Reversed Phase (RP HPLC)
Optimal combination with Mass Spectroscopy (MS)
Excellent stability

Product Overview

SeQuant® ZIC®-HILIC HPLC Columns

Merck KGaA, Darmstadt, Germany

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Merck KGaA, Darmstadt, Germany Manufacturer Website

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