Rotavirus Ag (stool) (EIA-3509)
The DRG Rotavirus Antigen Detection ELISA is an in vitro procedure for the qualitative determination of rotavirus antigen in feces. It is a double antibody (sandwich) ELISA using a polyclonal anti-rotavirus antibody to capture the antigen from the stool supernatant. A second anti-rotavirus monoclonal antibody is then added, which binds to the complex. This reaction is visualized by the addition of anti-mouse antibodies conjug…
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The DRG Rotavirus Antigen Detection ELISA is an in vitro procedure for the qualitative determination of rotavirus antigen in feces.
It is a double antibody (sandwich) ELISA using a polyclonal anti-rotavirus antibody to capture the antigen from the stool supernatant. A second anti-rotavirus monoclonal antibody is then added, which binds to the complex. This reaction is visualized by the addition of anti-mouse antibodies conjugated to peroxidase. The resulting blue color, following the addition of the chromogen and peroxide, indicates the presence of rotavirus antigens being bound by the anti-rotavirus antibodies.
